复合酶法提取甘草渣中总黄酮  

Composite Enzymatic Extraction of Total Flavonoids from Licorice Slag

作　　者：王芸芸[1] 李莉[1] 李香玉[1] 刘利军[1] 

机构地区：[1]宁夏大学化学化工学院,宁夏银川750021

出　　处：《化学与生物工程》2008年第8期49-51,共3页Chemistry & Bioengineering

基　　金：宁夏回族自治区自然科学基金资助项目(2007Zr060)

摘　　要：利用纤维素酶和果胶酶的破壁技术提取甘草渣中游离总黄酮,通过单因素实验,确定最佳酶法提取工艺为:固液比(质量体积比)1∶50,50 U.mL-1纤维素酶用量0.8 mL(每克甘草渣用量),120 U.g-1果胶酶用量30 mg(每克甘草渣用量),乙醇质量分数95%,温度50℃,酶解时间3 h,pH值4.5。与常规醇提法相比,游离总黄酮含量提高了1.15倍。Cellulase and pectinase enzyme broken technology was applied to extract free total flavonoids from licorice slag. Through single factor experiments, the optimal extraction process were determined as follows： ratio of solid to liquid 1 ： 50, 0.8 mL cellulase（50 U ·mL^1） per gram licorice slag, 30 mg pectinase（120 U·g^1 per gram licorice slag,ethanol mass fraction 95% temperature 50℃ , hydrolysis time 3 h, pH value 4.5. Compared with that of the conventional alcohol formulation, the content of free total flavonoids increased by 1.15 times of composite enzymatic extraction method.

关 键 词：酶法提取 纤维素酶 果胶酶 总黄酮 

分 类 号：Q814[生物学—生物工程] R284[医药卫生—中药学]