脑源性神经营养因子对出生后大鼠周围神经髓鞘化的作用  

Effect of endogenous BDNF on peripheral nerve myelination and its mechanism in postnatal rats

作　　者：石咏梅[1] 文晓丹[1] 李芳[1] 朱小京[2] 张建一[1] 

机构地区：[1]中南大学湘雅医学院人体解剖学和神经生物学系 [2]中南大学湘雅医学院,长沙410013

出　　处：《解剖学杂志》2008年第4期537-540,共4页Chinese Journal of Anatomy

基　　金：湖南省自然科学基金(JJY3051)

摘　　要：目的:探讨脑源性神经营养因子(BDNF)对出生后大鼠周围神经髓鞘化的作用及其作用机制。方法:用光镜和电镜方法检测坐骨神经纤维的髓鞘化情况;用免疫印迹方法检测坐骨神经p75的表达;用免疫荧光方法检测坐骨神经雪旺细胞内核转录因子(NF-κB)的表达。结果:出生后3 d,与对照组相比,实验组坐骨神经髓鞘化的神经纤维数目减少,p75表达下调,雪旺细胞内NF-κB转移率下调;出生后14 d,与对照组相比,实验组坐骨神经出现较多的髓鞘化异常。结论:内源性BDNF影响出生后大鼠周围神经的髓鞘化,尤其在早期作用明显;内源性BDNF主要通过p75和NFκ-B信号途径调节出生后大鼠周围神经的髓鞘化。Objective： To investigate the effect of endogenous brain-derived neurotrophic factor （BDNF） on the myelination of the postnatal peripheral nerve in rats. Methods.. The myelination of the sciatic nerve was measured with light microscope and electron microscope; the expression of p75 in the sciatic nerve was measured by Western blot; the nuclear transiocation of NF-kappa B in the sciatic nerve were showed by immunofluorescence. Results ： At postnatal day 3 （P3）, the number of my- elinating fibres, the p75 expression and the rate of NF-kappa B nuclear translocation in the sciatic nerve were reduced in the anti-BDNF group compared to those in the control group; at P14, the number of abnormal myeiinating fibres in the sciatic nerve was increased in the anti-BDNF group compared to that in the control group. Conclusion： Endogenous BDNF affected my：elination of postnatal peripheral nerve through p75 and NF-kappa B signal transduction pathway.

关 键 词：发育 脑源性神经营养因子 P75 NF-KB 髓鞘化 

分 类 号：R741[医药卫生—神经病学与精神病学]