烟草病程相关蛋白PR-1a的三维模型和荧光光谱  

Homology Modeling and Fluorescence Spectra Study of Tobacco Pr-1a

作　　者：李佳[1] 郑清川[2] 杜林方[1] 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,四川成都610064 [2]吉林大学理论化学研究所理论化学计算国家重点实验室,吉林长春130023

出　　处：《应用与环境生物学报》2008年第4期466-468,共3页Chinese Journal of Applied and Environmental Biology

基　　金：教育部新世纪人才资助计划(NCET-04-0861);四川大学985计划项目~~

摘　　要：利用同源模建和分子动力学模拟方法搭建了烟草病程相关蛋白PR-1a的三维结构,在此基础上预测出PR-1a具有2个可能的活性位点,进一步分析PR-1家族特异保守序列,结果显示位点1的可能性更大.利用蛋白质内源荧光为探针,对分离的重组表达PR-1a蛋白进行了热稳定性研究.低于60℃处理,PR-1a蛋白内源荧光只略微降低;70℃处理30min引起PR-1a蛋白内源荧光明显降低,结果显示,PR-1a中色氨酸残基主要处于蛋白质分子内部,这与三维结构模型预测结果相符.Tobacco PR-1a model was obtained with the aid of the homology modeling and molecular dynamics methods, and was found reliable in Profile-3D and ProStat assessments. On the basis of this model, the omponents and structure of the active sites in tobacco PR-1a were analyzed. Further analysis of the conserved sequence in PR-1 family suggested that site 1 was likely more active than site 2. Then, fluorescence spectra were used as the probe to investigate the thermal stability of purified recombinant PR-1a proteins. The fluorescence intensity of PR-1a was reduced slightly when treated at 20-60 ℃, but it was obviously weakened at 70 ℃ for 30 minutes. The results indicated that tryptophan residues were mostly at the interior of PR-1a protein, which was consistent with the final 3D model. Fig 5, Ref 15

关 键 词：病程相关蛋白 分子动力学 同源模建 荧光发射光谱 热稳定性 

分 类 号：S432.1[农业科学—植物病理学]