海洋石油降解菌的筛选与降解特性  

Isolation of Marine Oil-degrading Bacteria and Their Biodegradation Characteristics

作　　者：苏莹[1] 陈莉[1] 汪辉[1] 刘兆普[1] 

机构地区：[1]南京农业大学资源与环境学院,江苏省海洋生物重点实验室,南京210095

出　　处：《应用与环境生物学报》2008年第4期518-522,共5页Chinese Journal of Applied and Environmental Biology

基　　金：国家"863"海洋生物技术计划(2003AA627040)资助~~

摘　　要：用采自山东胜利油田的石油污染水体,以原油为唯一碳源,经人工海水培养基富集培养,得到10株细菌.通过降解率的测定,发现其中一株细菌HB-1具有较强的石油降解能力,200r/min振荡培养6d,其原油的降解率达54·74%.根据菌落特征、菌体形态、生理生化特性和16SrDNA序列分析,确定HB-1为不动杆菌属(Acinetobactersp.).进一步实验表明,该菌株最适宜生长和降解石油的条件为:温度25～32℃,初始pH值6·5～7·5,盐度3%,此条件符合实际海洋环境的要求.By enriching manual marine culture with crude oil as a sole carbon source, 10 strains of bacteria were obtained from oil contaminated water samples which were collected from the Shengli Oil Field in Shandong. Through measuring the degradation rates of the 10 bacterial stains, strain HB-1 had the highest oil-degradation capacity and its degradation rate was 54.74% after it was incubated at shaking rate 200 r/min for six days. It was identified as Acinetobacter sp. by its morphologic, physiological and biochemical characteristics, especially by the analysis of 16S rDNA sequences. Its optimal conditions for growth and degradation include temperature at 25 -32 ℃, pH 6. 5 -7. 5 and salinity 3%. These conditions accord with the requirements of the actual marine environment. It＇s estimated that strain HB-1 is of potential in bio-treatment of marine oil pollutants. Fig 6, Tab 2, Ref 22

关 键 词：海洋石油降解菌 菌株筛选 16S RDNA 生物降解 

分 类 号：X172[环境科学与工程—环境科学]