不同级别胶质瘤细胞系与侵袭和转移相关的重叠基因  

Identification of invasion-and metastasis-related overlapped genes in different grade glioma cell lines

作　　者：曾义[1] 杨忠[2] 韩杨云[1] 游潮[3] 

机构地区：[1]德阳市人民医院神经外科,四川德阳618000 [2]第三军医大学神经生物学教研室,重庆400038 [3]四川大学华西医院神经外科,四川成都610041

出　　处：《西部医学》2008年第5期908-910,共3页Medical Journal of West China

基　　金：四川省卫生厅科研项目(NO.050209)

摘　　要：目的利用cDNA微阵列分析不同级别和全反式维甲酸诱导的胶质瘤细胞系与侵袭和转移相关的重叠基因,为进一步研究胶质瘤的侵袭机制提供依据。方法通过cDNA微阵列技术比较两种不同级别胶质瘤细胞系CHG-5和SHG-44以及全反式维甲酸诱导3天的SHG-44细胞系的基因表达谱,消除细胞系间的个体差异,鉴定与胶质瘤侵袭和转移相关的重叠基因。随机选择数个重叠基因进行Northern杂交实验,验证cDNA微阵列结果的可靠性。结果实验鉴定出31个重叠基因,表达上调20个,下调11个。其中,4个重叠基因,包括CD151、G3BP、UGB和CSTB与胶质瘤的侵袭和转移相关。部分重叠基因的可靠性为Northern杂交实验所验证。结论初步揭示不同级别的胶质瘤细胞系之间存在侵袭和转移相关基因的差异表达,提示这些重叠基因可能与胶质瘤的进展有关。Objective To identify the differentially expressed overlapped genes related to tumor invasion and metastasis from different grades and all-trans retinoic acid treated glioma cell lines with cDNA microarray, and to provide basic data for further research on mechanism of tumor invasion in human gliomas. Methods cDNA microarray was applied to compare the gene expression profiling between two different grades glioma cell lines （CHG-5, SHG-44） and all-trans retinoic acid （ATRA） treated SHG-44 for 3 days. Some overlapped genes related to tumor invasion and metastasis were obtained. To confirm the results of genes detected with the cDNA microarray, some differentially expressed overlapped genes were randomly selected for Northern blot analysis. Results Thirty-one overlapped genes were identified with cDNA microarray. The four overlapped genes, including CD151, G3BP, UGB, and CSTB, were involved in tumor invasion and metastasis. Validation of some differentially expressed overlapped genes was performed with Northern blot. Conclusion The results reveal that there is differential expression of invasion- and metastasisrelated genes in different grades glioma cell lines, which suggests that the above-mentioned overlapped genes might be associated with progression of gliomas.

关 键 词：转移 CHG-5 SHG-44 胶质瘤 全反式维甲酸 CDNA微阵列 

分 类 号：R739.4[医药卫生—肿瘤]