毛细管电泳同时分离测定阿魏酸、异阿魏酸的研究  

Studies on separation and determination of ferulic acid and isoferulic acid by capillary electrophoresis

作　　者：李利军[1] 冯军[2] 陈其锋[2] 吴峰敏[2] 钟招亨[2] 孔红星[1] 吴健玲[2] 

机构地区：[1]广西工学院生物与化学工程系,柳州545006 [2]广西大学化学与化学工程学院,南宁530005

出　　处：《分析试验室》2008年第9期46-49,共4页Chinese Journal of Analysis Laboratory

基　　金：广西自然科学基金(桂科自No.0481019);广西教育厅科学研究基金(桂教科[2000]392)项目资助

摘　　要：建立了同时分离测定阿魏酸、异阿魏酸的毛细管电泳（CZE）新方法。以20mmol／L硼砂为背景电解质，体积分数15％异丙醇为有机改性剂，分离电压为20kV，在219nm波长下紫外检测。对硼砂浓度、有机溶剂体积分数、分离电压等因素对分离的影响做了系统的研究，最后确立了阿魏酸、异阿魏酸的最佳分离条件。阿魏酸、异阿魏酸分别在2．40-24．0μg／mL、1．80～18．0μg／mL范围内线性关系良好（r=0．9995和r=0．9991），回收率分别为96．61％～101．9％，98．80％～101．8％。方法已用于升麻中阿魏酸、异阿魏酸的测定。A new method for the simultaneous determination of ferulic acid and isoferulic acid in Cimicfugafoetida by capillary eleetrophoresis has been proposed. The operating buffer was composed of 20 mmol/L disodium tetraborate deeahydrate and 15% （V/ V） isopropanol. The applied voltage was 20 kV and the UV detection wavelength was 219 nm. The linear ranges of the method were 2.40 - 24.0μg/mL （ r = 0.9995） for ferulic acid and 1.80 - 18.0μg/mL （r = 0.9991）for i soferulic acid. The recoveries were 96.61% - 101.93 % and 98.80% - 101.78 % respectively. Tiffs method is simple, rapid and accurate. It had been successfully used tor the determination of ferulic acid and isoferulic acid in Cimicfuga foetida.

关 键 词：毛细管电泳 阿魏酸 异阿魏酸 升麻 

分 类 号：O557.8[理学—热学与物质分子运动论]