PPAR-γ偶联的信号通路可能参与大鼠肝再生  

PPAR-γ coupled signaling pathways involved in rat liver regeneration

作　　者：徐存拴 唐自阔 

机构地区：[1]河南师范大学生命科学学院 [2]河南省科技部共建细胞分化调控重点实验室,河南新乡453007

出　　处：《基础医学与临床》2008年第8期810-815,共6页Basic and Clinical Medicine

基　　金：973计划前期研究专项(2006CB708506)

摘　　要：目的了解PPAR-γ偶联的信号通路在大鼠肝再生(LR)中作用。方法用搜集网站资料和查阅相关论文等方法获得上述通路相关基因,用RatGenome 230 2.0芯片检测它们在大鼠肝再生中表达情况,用真手术与手术对照比较方法确定肝再生相关基因。结果初步证实上述基因中64个基因与肝再生相关。肝再生启动、G0/G1过渡、细胞增殖、细胞分化和组织结构功能重建等4个阶段起始表达的基因数为28、4、34和2,基因总表达次数为72、41、247和90,表明相关基因主要在肝再生启动阶段起始表达,在不同阶段发挥作用。它们共分为11种表达方式,表明肝再生中这些基因表达变化多样和复杂。结论PPAR-γ偶联的信号通路在肝再生早期、前期和后期促进糖元合成;在整个肝再生中抑制炎症反应,促进细胞增殖和迁移。Objective To investigate the possible contribution of PPAR-γ coupled signaling pathways to rat liver regeneration （LR） at transcription level. Methods The above signaling pathways-related genes were obtained by collecting database data and retrieving pertinent literatures. The gene expression during LR was checked by Rat Gehome 230 2.0 array, and LR-associated genes were identified by comparing difference between partial hepatectomy （PH） and operation control （OC） groups. Results Sixty four genes were found to be LR-associated. The number of initially and totally expressed genes occurring in initiation phase of LR, G0/G1 transition, cell proliferation, cell differentiation and structure-function reconstruction was 28, 4, 34, 2 and 72, 41,247, 90 respectively. Classification of their expression patterns into 11 groups reflected diversity and complexity of gene expression alteration in LR. Conclusion PPAR-γ coupled signaling pathways may promote glycogen synthesis in forepart, prophase and anaphase of LR, cell proliferation and migration in the whole LR, while inhibits inflammation during LR.

关 键 词：部分肝切除 RAT GENOME 230 2.0芯片 PPAR-γ偶联的信号通路 肝再生相关基因 

分 类 号：R575[医药卫生—消化系统]