小鼠梗阻性黄疸模型的建立及动态观察  

Establishment of a mouse model of biliary obstruction and its dynamic observations

作　　者：张守华[1] 廖彩仙[1] 张春兴[1] 邓红[2] 朱航[2] 雷蕾[2] 药晨[3] 

机构地区：[1]南方医科大学南方医院肝胆外科,广东广州510515 [2]南方医科大学公共卫生与热带医学学院营养卫生学系,广东广州510515 [3]南方医科大学南方医院泌尿外科,广东广州510515

出　　处：《南方医科大学学报》2008年第9期1579-1581,共3页Journal of Southern Medical University

基　　金：国家自然科学基金(30371385);广东省自然科学基金(04020419)

摘　　要：目的建立小鼠梗阻性黄疸模型,并对该模型进行动态观察。方法选取Balb/c小鼠64只,随机等分为模型组(56只)和正常对照组(8只),实验组开腹后游离出胆总管,双重结扎,然后关腹。于实验的第12 h,1、2、3、4、5、7 d动态观察胆总管直径及白细胞总数、淋巴细胞总数、中间细胞总数及淋巴细胞总数百分数和中间细胞总数百分数,测血清总胆固醇、丙氨酸氨基转氨酶、天冬氨酸氨基转氨酶、碱性磷酸酶、总胆红素、直接胆红素及间接胆红素值。取动物肝脏进行病理组织学检查。结果实验结果表明,小鼠随着胆总管结扎时间的延长,其胆总管的直径、白细胞总数、淋巴细胞总数、中间细胞总数及百分数、肝功能相关酶指标等,都存在一定的曲线变化。结论Balb/c小鼠在通过结扎胆总管造成阻塞性黄疸时,其变化的规律与人类相似,并且造模时间短(只需2 d),是研究梗阻性黄疸病理生理机制和治疗的可靠实验模型。Objective To establish a mouse model ofbiliary obstruction. Methods Sixty-four Balb/c mice were divided into experimental group and control group. Obstructive jaundice was induced in the mice in the experimental group by common bile duct ligation. The level of the common bile duct diameter, WBC, LYM MID, LYM%, MID% and ALT, AST, TBIL, DBIL, IBIL, ALP and CHOL were measured 12 h and l, 2,3, 4, 5, and 7 days after the ligation. The morphological changes in the liver were also observed. Results The level of common bile duct diameter, WBC, LYM, MID, LYM%, MID% and ALT, AST, TBIL, DBIL, ALP and CHOL all underwent changes with time following certain patterns. Conclusion The jaundice manifestation of this model is similar to that of patients with biliary obstruction, and this model may provide a reliable model for studying the mechanism of obstructive jaundice.

关 键 词：梗阻性黄疸 模型 小鼠 动态观察 

分 类 号：R-332[医药卫生] R657.4