毛细管电泳法分离检测多酶水解物中的大豆多肽  

Seperation and Examination Soybean Polypeptides of Enzymes Hydrolysis Products with Capillary Electrophoresis

作　　者：刘静[1] 

机构地区：[1]咸阳师范学院化学系,陕西咸阳712000

出　　处：《咸阳师范学院学报》2008年第4期44-46,共3页Journal of Xianyang Normal University

基　　金：陕西省教育厅专项基金项目(06JK166;07JK420);咸阳师范学院科研基金项目(06XSYK107)

摘　　要：采用高效毛细管电泳对酶水解得到的大豆多肽进行分离检测。实验表明:高效毛细管电泳法是分离检测大豆多肽的有效方法。最佳分离检测条件:100mmol/LTris/HCl(pH=8.5)+3.5mmol/LSDS+0.1%PEO为缓冲液,进样时间10s,运行电压18kV,气动进样,用HPCE以无胶筛分方式,成功将大豆多肽进行了分离和检测。Soybean polypeptides of enzymes hydrolysis products was separated and tetsed hy high performance capillary electrophoresis（HPCE） in the paper. The results showed that HPCE was a highly effective way of seperation and examination.The optimized conditions is：the running buffer was composed of 100mmol/L Tris / HCI（pH=8.5） ＋ 3.Smmol/L SDS＋0.1%PEO and the running voltage was 18kV, time of entering sample is 10s, by the way of non-gel sieving high-performance capillary electrophoresis, seperation and examination was carried up successfully.

关 键 词：大豆多肽 酶水解 无胶筛分 毛细管电泳 

分 类 号：O657.7[理学—分析化学]