肝细胞癌组织中Glypican-3基因表达及其调控机制  

Expression of Glypican-3 gene and its regulating mechanism in hepatocellular carcinoma

作　　者：缪辉来[1] 邱志东[1] 温继育[2] 谢贵林[1] 陈念平[1] 陈明[1] 

机构地区：[1]广东医学院附属医院肝胆外科,广东湛江524001 [2]广东医学院附属医院肿瘤科,广东湛江524001

出　　处：《中国病理生理杂志》2008年第9期1730-1733,共4页Chinese Journal of Pathophysiology

基　　金：广东省科技计划资助项目(No.2004B30301001);湛江市科技招标资助项目(No.20040025)

摘　　要：目的:探讨肝细胞癌中Glypican-3(GPC3)基因表达及调控机制。方法:采用RT-PCR和聚合酶链反应-单链构象多态性(PCR-SSCP)方法分别检测48例肝细胞癌的癌组织、39例癌旁组织和31例正常肝组织中GPC3 mRNA的表达和基因突变;免疫组化S-P法检测GPC3、P53和PCNA蛋白的表达。结果:GPC3 mRNA在肝细胞癌的癌组织中的阳性表达率为77.1%,但其在癌旁和正常肝组织中不表达;肝细胞癌的癌组织中未发现GPC3基因突变;GPC3与P53蛋白表达无相关性(r=-0.12574,P>0.05),肝细胞癌的癌组织中GPC3表达阳性和阴性的增殖细胞核抗原(PCNA)平均指数分别为(46.32±27.54)%、(39.83±21.47)%,P>0.05。结论:肝细胞癌组织中GPC3 mRNA高表达与基因突变无关,没有直接影响P53和PCNA蛋白表达。AIM： To investigate the expression of Glypican -3 gene and its regulating mechanism in hepatocellular carcinoma. METHODS ： The expression of GPC3 mRNA and its gene mutation in 48 hepatocellular carcinoma tissues, 39 paracarcinomatous tissues and 31 normal liver tissues were detected by using RT - PCR and PCR - SSCP. The expressions of GPC3 protein, P53 and PCNA protein were detected by using immunohistochemistry S-P. RESULTS： The positive expressive rate of GPC3 mRNA was 77. 1% in hepatocellular carcinoma tissue. No expression of GPC3 mRNA in paracarcinomatous tissues and normal liver tissues was observed. No gene mutation of GPC3 in hepatocellular carcinoma tissue was found. No correlation was found between GPC3 and P53 （ r = - 0. 12574, P 〉 0. 05 ）. The mean index of proliferating cell nuclear antigen （PCNA） in positive and negative GPC3 expression were （46. 32 ± 27.54）% and （39. 83 ± 21.47 ） % , respectively （ P 〉 0. 05 ）. CONCLUSION ： The high expression of GPC3 mRNA in hepatocellular carcinoma is independent to the gene mutation, and to the expression of P53 and PCNA protein.

关 键 词：癌 肝细胞 GLYPICAN-3 基因突变 P53 增殖细胞核抗原 

分 类 号：R363[医药卫生—病理学]