非水反相高效液相色谱-气相色谱法分析苏子油甘油三酯的组成  

Analysis of Triacylglycerol Composition of Perilla Oil by Combination of Non Aqueous Reversed Phase High Performance Liquid Chromatography with Gas Chromatography

作　　者：崔凯[1] 丁霄 霖肖刚 戴军 

机构地区：[1]无锡轻工大学食品工程系

出　　处：《色谱》1997年第6期470-473,共4页Chinese Journal of Chromatography

摘　　要：苏子油是目前已知α－亚麻酸含量最高的植物物种。首次对苏子油甘油三酯主要组分的组成结构进行了研究。非水反相高效液相色谱和气相色谱的结合为油脂中甘油三酯的分离分析提供了一个简便准确的方法。Perilla oil was verified to be a rich source of polyunsaturated fatty acid(PUFA). Its linolenic content is 6482%, the highest in the plants. Its triacylglycerol(TAG) components were isolated and identified first time in this paper by a combination of non aqueous reversed phase high performance liquid chromatography(RP HPLC) with gas chromatography(GC). The TAG of perilla oil were isolated by HPLC with a Zorbax ODS column(5μm,46mm i. d.×250mm; Dupont, Inc) and differential refractometer. The eluent was acetone/acetonitrile (80∶20,V/V) with flow rate of 10mL/min. The TAG component acyl groups were converted to fatty acid methyl esters(FAME). The acyl constituents for each TAG were determined by GC analysis. Gas chromatography of FAMEs was performed with a AC20 Carbowax 20M column(30m×032mm i. d.). Detector and injection port temperatures were 260℃. Column temperature was programmed from 120℃, 1min initially hold, then 8℃/min to 220℃ and finally hold for 10min. Nitrogen was the carrier gas. FAMEs peaks were identified by comparison of their retention times with those of standards. The retention times of the palmitic, stearic, oleic, linoleic, n nonadecanoic acid(internal standard)and linolenic FAMEs were 107,130,133,138,142 and 147min respectively. Five main components were determined. They were LnLnLn(3410%), LnLnL(1322%),LnLL(622%),LnLnO(1138%) and LnLnP(917%). The TAG contents were corrected from their fatty acids content. The combination of non aqueous RP HPLC with GC is a simple and rapid method for the analysis of oil TAG structure and is also very helpful for the identification of oil quality.

关 键 词：HPLC GC 苏子油 甘油三酯 

分 类 号：TS225.19[轻工技术与工程—粮食、油脂及植物蛋白工程] TS227[轻工技术与工程—食品科学与工程]