固定化酶不对称合成(S)-(+)-布洛芬的研究  

Study on Asymmetric Synthesis of ( S ) (+) Ibuprofen by Immobilized Enzyme

作　　者：吉鑫松[1] 许文光 钱雪明[1] 袁中一[1] 李仁宝 刘会军 赵国骏[3] 

机构地区：[1]中国科学院上海生物化学研究所 [2]山东新华制药厂 [3]扬州大学农学院

出　　处：《分子催化》1997年第6期417-420,共4页Journal of Molecular Catalysis(China)

摘　　要：能够不对称水解布洛芬乙酯的酵母菌Ｔ１５８（ＴｒｉｃｈｏｓｐｏｒｏｎｃｕｔａｎｅｕｍＴ１５８）固定化在壳聚糖珠中，固定化酵母菌的活力回收达６０％，最适温度为４５℃（游离酵母为３５℃），半衰期为７５ｄ以上．脂肪酶（Ｃａｎｄｉｄａｃｙｌｉｎｄｒａｃｅａ）也固定化在中空纤维反应器中，利用该反应器水解外消旋布洛芬乙酯得到的（Ｓ）－（＋）－布洛芬，其ｅ．ｅ．值为８０％．The yeast cells T158 ( Trichosporon cutaneum T158) that could asymmetrically hydrolyze ethyl ibuprofenate to ( S ) (+) ibuprofen were entrapped into chitasan beads. The activity recoveries were 60% for immobilized yeast cells. The optimum temperature for the immobilized cells is 45℃, but is 35℃ for free cells, the half life of the immobilized cells is more than 75 days. The lipase from candida cylindracea were also immobilized into hollow fibre reactor. By using this reactor, 80% of enantiometric excess of ( S ) (+) ibuprofen was obtained through the asymmetric hydrolysis of racemic ethyl ibuprofenate.

关 键 词：固定化酶 固定化酵母 布洛芬 (S)-(+)-布洛芬 

分 类 号：R971.1[医药卫生—药品]