石棉肺癌组织中p53基因突变的PCR-SSCP及部分序列分析研究  

PCR SSCP and Sequencing Analysis on Mutations of Anti oncogene p53 in Asbestos Related Lung Cancer

作　　者：付德辰[1,2,3] 刘秉慈[1,2,3] 缪庆[1,2,3] 王海华[1,2,3] 尤宝荣[1,2,3] 罗朝中[1,2,3] 

机构地区：[1]中国预防医学科学院劳动卫生与职业病研究所 [2]中国科学院生物物理所 [3]四川省劳动卫生监督所

出　　处：《卫生研究》1997年第5期289-292,共4页Journal of Hygiene Research

基　　金：中国预防医学科学院科研基金

摘　　要：为研究石棉致癌的分子机理，本研究首次直接分析石蜡包埋的石棉肺癌病例肺组织中抗癌基因ｐ５３的突变情况。利用聚合酶链反应－单链构象多态性（ＰＣＲ－ＳＳＣＰ）技术分析１０例石棉肺癌病例，检测到７例（８个片段）存在ｐ５３基因的突变，突变位点位于其第５、７和８外显子区内，突变率为７０％。对其中２例进行ＰＣＲ产物直接序列分析，发现其基因一级结构分别发生Ｃ→Ａ和Ｔ→Ａ转换。To explore the carcinogenic effect of asbestos at molecular level, we directly analyzed the mutations of exon 5,7 and 8 of anti oncogene p53 in paraffin embedded human lung tissue affected by asbestos for the first time. Mutations of p53 gene located in exon 5,7 and 8 were detected in 7 of 10 cases by polymerase chain reaction single strand conformation polymorphism (PCR SSCP) analysis. The mutation rate found in this study is 70%. Two cases were analyzed by PCR product direct sequencing and C→A and T→A transformations were detected. Mutation of p53 gene may be one of the characteristics of asbestos related lung cancer.

关 键 词：肺肿瘤 石棉肺癌 P53基因 基因突变 PCR-SSCP 

分 类 号：R734.202[医药卫生—肿瘤]