人工合成小麦Am3大穗多粒QTL的发掘与利用  

Mapping of Large-Spike and Much-Kernel QTL by Using a Synthetic Wheat Am3 as Donor

作　　者：王瑾[1] 廖祥政[2] 杨学举[1] 周荣华[2] 贾继增[2] 

机构地区：[1]河北农业大学农学院,保定071001 [2]中国农业科学院作物科学研究所/农业部作物种质资源与生物技术重点开放实验室,北京100081

出　　处：《植物遗传资源学报》2008年第3期277-282,共6页Journal of Plant Genetic Resources

基　　金：国家"973"计划项目(G1998010205)

摘　　要：穗粒数是小麦的重要产量性状之一，本研究以人工合成双二倍体小麦Am3为供体，普通小麦品种莱州953为受体，培育出了高穗粒数BC5F1导入系，以导入系后代75个BC5F1为材料，利用复合区间作图法对其进行穗部性状的QTL定位。共检测到2个控制穗长、4个控制小穗数、2个控制穗粒数的QTL位点，贡献率分别为1％～22％、1％～9％和1％～15％。其中穗长和穗粒数分别有1个QTL能在两年重复检测到。并且在1A染色体上检测到同时控制小穗数和穗粒数的QTL，穗长和小穗数的QTL被定位在4A染色体上同一个区域，表明这2个位点是与穗部性状有关的热点区域。本研究发现的QTL多为来自Am3的新位点，对于小麦改良将具有重要价值。Grain number is an important yield trait in wheat. A BC5F1 population consisting of 75 plants was constructed by selfing one of the BC5F1 plant, which were generated from repeated backcrossing between the exotic hexaploid wheat genotype Am3 and the common wheat genotype Laizhou 953. Composite interval mapping was used to detect QTL for three spike characteristics. In total, 2, 4 and 2 putative QTL were detected for spike length, spikelets and grain number per spike, and can explain 1% -22% ,1% -9% and 1% -15% phenotypic variation, re- spectively. One spike length QTL and one grain number per spike QTL were detected in two seasons. The QTL in 1A for spikelets and grain number per spike were mapped in the same interval. The QTL in 4A for spike length and spikelets were mapped in the same interval. The result suggested that this 2 loci were associated with spike characteristics, it may be ＂hot spot＂ for major loci controlling such traits. Most of the QTL which were from synthetic wheat Am3 were new QTL, and it may be important to improve wheat yield.

关 键 词：小麦 穗部性状 QTL 人工合成小麦 导入系 

分 类 号：S512.1[农业科学—作物学]