β-环糊精与青蒿素类药物非共价复合物的电喷雾质谱研究  

Investigation of Noncovalent Complexes Between β-Cyclodextrin and Artemisinin by Electrospray Ionization Mass Spectrometry

作　　者：李娟[1] 徐海伟[1] 王少敏[2] 李华雨[2] 韩冬[2] 刘宏民[1] 

机构地区：[1]郑州大学药学院 [2]郑州大学化学系

出　　处：《分析化学》2008年第9期1212-1216,共5页Chinese Journal of Analytical Chemistry

摘　　要：采用电喷雾飞行时间质谱(ESI-TOF-MS)技术,对β-环糊精与3种青蒿素类药物所形成的复合物进行研究,在正离子检测方式下,将β-环糊精与青蒿素类药物等体积比混合后直接进样,然后利用源内碰撞诱导解离(CID)技术对其复合物进行分析。实验表明,在气相中,该非共价复合物可以稳定存在,其化学计量比分别为1:1和2:1。运用该法测定了1:1包络物的结合常数,考察了质谱条件及溶液条件对形成包络物的影响,通过比较结合常数的大小,探讨了β-环糊精与青蒿素类药物在气相状态下的作用方式。To investigate the interactins of non-covalent complexes between β-cyclodextrin and types of three artemisinis, electrospray ionization-time of flight mass spectrometry （ESI-TOF-MS） was used to detect equiv- olume mixtures of β-cyclodextrin and artemisinins in positive ion mode. In this study, the samples were directly injected into electrospray ionization system. A method used of collision induced dissociation （CID） was applied for the study of their complexes, the results indicated that the non-covalent complexes can stably exist in gas phase, and the 1：1 and 2：1 binding modes were indicated by examining the binding stoichiometry from ESI mass spectra. With electrospray ionization-time of fight-mass spectrometry, the binding constants （K） of the 1 ： 1 inclusion complexes were determined respectively, and the parameters of mass spectrometry and solu- tion system were optimized and investigated. By comparison of the K values, the interactions between β-cyclo- dextrin and artemisinins in the gas phase were discussed.

关 键 词：非共价复合物 青蒿素类药物 Β-环糊精 结合常数 电喷雾飞行时间质谱 

分 类 号：R284[医药卫生—中药学]