猪皮胶原蛋白的提取及其结构表征  

Extraction and characterization of collagen from pigskins

作　　者：周玉惠[1] 叶正涛[1] 肖立芳[1] 刘海[1] 孙艳斌[1] 胡小方[1] 

机构地区：[1]湖北大学材料科学与工程学院,湖北武汉430062

出　　处：《湖北大学学报（自然科学版）》2008年第3期287-289,共3页Journal of Hubei University：Natural Science

摘　　要：采用较简便的酶法从猪皮中提取胶原蛋白,通过SDS-PAGE凝胶电泳、紫外光谱定性分析,证明所提取胶原蛋白为Ⅰ型胶原蛋白,计算得分子量约为320 kD.利用FTIR、DSC进一步测试,实验结果表明胶原蛋白具有完整的三螺旋结构,即具有生物活性.Type Ⅰ collagen was extracted from pig-skin by acid-pepsin digestion method. UV and SDS-PAGE analysis suggest that the extraction product is type Ⅰ collagen, and its molecule mass is about 320 kDa. FTIR and DSC analysis can confirm that the collagen have the integrated triple helix structure, which means the collagen own bioactivity.

关 键 词：Ⅰ型胶原蛋白 猪皮 傅立叶红外 差示量热扫描仪 凝胶电泳 

分 类 号：O636.9[理学—高分子化学] Q599[理学—化学]