西洛他唑对糖尿病视网膜病变中血小板反应素表达影响的研究(英文)  

THE EFFECT OF CILOSTAZOL ON EXPRESSION OF THROMBOS PONDIN-1 IN DIABETIC RETINOPATHY

作　　者：王剑勇[1] 顾扬顺[1] 严丽萍[1] 滕晓东[1] 张晓明[2] 

机构地区：[1]浙江大学医学院附属第一医院,杭州310003 [2]浙江大学医学院,杭州310006

出　　处：《分子细胞生物学报》2008年第4期301-308,共8页Journal of Molecular Cell Biology

基　　金：浙江省自然科学基金资助,项目编号:2007C3306~~

摘　　要：探讨西洛他唑对糖尿病视网膜病变病理生理中的影响及可能的作用机制。用链脲佐菌素(STZ)腹腔注射建立糖尿病模型,用免疫组织化学法、实时荧光定量PCR法观察西洛他唑治疗组、糖尿病对照组和正常对照组视网膜中血小板反应素(TSP-1)的变化。每隔1周测量血糖一次,4周后糖尿病治疗组、糖尿病组与正常组大鼠的体重、血糖有显著性差异(P<0.01);糖尿病治疗组、糖尿病对照组和正常对照组间TSP-1表达有显著性差异(P<0.01)。在早期糖尿病大鼠的视网膜神经节细胞层、内核层中均有明显的TSP-1表达,糖尿病西洛他唑治疗组视网膜中TSP-1的表达要低于糖尿病对照组。实验显示西洛他唑可能通过阻止TSP-1的过度表达,在一定程度上延缓其糖尿病视网膜病变的发生。To explore the effect of cilostazol in the pathophysiology of diabetic retinopathy and its mechanism, we intraperitoneal injection streptozotocin （STZ） to induce rats diabetic model to study the alteration of the thrombospondin-1 （TSP-1） in the retina of diabetic rats, cilostazol treatment diabetic rats and normal rats by immunohistochemistry, real-time quantitative reverse transcription-polymerase chain reaction. The weight, blood sugar and urine sugar were also measured before and after model induction of these three groups. The data of weight, blood sugar and urine sugar indicated no significant difference in these three groups before diabetes induction. Four weeks after the injection of STZ, the weight, blood sugar and urine sugar had significant difference among these three groups （P〈 0.01）. When compared with the normal retina, TSP-1 expression was increased in the diabetic rat＇s retina, as shown by increased optical density and immunohistochemistry positive cell number but this was not serious in cilostazol treatment rats （P〈0.01）. Our study confirmed that up-regulation of TSP-1 expression in retina of streptozotocin-induced diabetic rats under hyperglycemia condition and cilostazol treatment could prevent TSP-1 overexpression. This indicates a protective way in the pathophysiology of diabetic retinopathy.

关 键 词：糖尿病视网膜病变 西洛他唑 血小板反应素-1 

分 类 号：R774.1[医药卫生—眼科]