AQP-9在大鼠感染性脑水肿脑组织中的表达及意义  

Expression of aquaporin-9 in the brain tissue of rats with infectious brain edema

作　　者：田培超[1] 王怀立[1] 罗强 禚志红[1] 

机构地区：[1]郑州大学第一附属医院儿科,郑州450052

出　　处：《中华神经医学杂志》2008年第9期919-922,共4页Chinese Journal of Neuromedicine

摘　　要：目的探讨水通道蛋白-9（AQP-9）在内毒素脂多糖（LPS）致大鼠感染性脑水肿脑组织中的表达及意义。方法1月龄普通级SD大鼠128只采用随机数字表法分为生理盐水（NS）组（64只）和LPS组（64只）．采用颈内动脉注射LPS制作大鼠感染性脑水肿模型，模型成功后每组均选取6h、12h、24h和48h4个时间点．在不同时间点采用HE染色观察脑组织形态学改变；干湿重法测定脑组织含水量（BWC）；甲酰胺法测定脑组织伊文思蓝（EB）含量；免疫组织化学法检测脑组织AQP-9蛋白的表达量：采用逆转录多聚酶链反应（RT—PCR）方法检测AQP-9 mRNA的表达水平并对结果进行相关性分析。结果HE染色结果显示LPS组血管周围间隙增宽、炎性细胞浸润、胶质细胞体积增大肿胀、神经元空泡变性、细胞核固缩等。与NS组相比，LPS组6h、12h、24h和48h BWC、EB含量、AQP-9蛋白及AQP-9mRNA表达水平均增高，差异具有统计学意义（P〈0．05）。同时LPS组BWC和EB含量、AQP-9蛋白、AQP-9mRNA表达量、AQP-9mRNA表达量与EB含量、AQP-9蛋白与mRNA表达量均呈正相关。结论AQP-9可能参与感染性脑水肿的发生和发展。Objective To investigate the expression of aquaporin-9 （AQP-9） in the brain tissue of rats with infectious brain edema and explore the role of AQP-9 in the occurrence and progression of the brain edema. Methods A total of 128 normal 1-month-old SD rats weighing 70-100 g of either sex were randomly divided into two equal groups, namely the normal saline （NS） group and lipopolysaccharide （LPS） group. Acute infectious brain edema was induced in rats in the LPS group by injecting LPS via the left internal carotid artery, and the rats in the NS groups received NS injection. At 6, 12, 24 and 48 h after the injection, the brain tissue was taken from the rats to observe the histopathology by Hematoxylin-Eosin Stain and measure the brain water content （BWC）. The permeability of the blood-brain barrier of the rats was tested using Evans blue （EB） method. The expressions of AQP-9 protein and mRNA in the brain tissue were detected using immunohistochemistry and RT-PCR, respectively. Results In LPS group, the space around the blood vessels was obviously broadened in the brain tissue, where inflammatory cell infiltration, gliocyte swelling, vacuolar degeneration of the neurons and neuronal nuclear shrinkage were seen. At 6, 12, 24, and 48 h following LPS or NS injection, the BWC, EB content, and expressions of AQP-9 protein and mRNA in the LPS group were all significantly higher than those in the NS group （P〈0.05）. In the LPS group, positive correlations were found between BWC and EB content, AQP-9 protein expression and BWC, AQP-9 mRNA and BWC, AQP-9 mRNA and EB content, and between AQP-9 protein and mRNA. Conclusion AQP-9 might participate in occurrence and development of infectious brain edema in rats, it might be positive correlation.

关 键 词：水通道蛋白-9 感染性脑水肿 逆转录多聚酶链反应 

分 类 号：R741[医药卫生—神经病学与精神病学]