一株耐热石油烃降解菌的细胞疏水性及乳化、润湿作用研究  

Cell-surface Hydrophobicity, Emulsification and Wetting Property of a High Temperature Hydrocarbon-degrading Strain

作　　者：张明露[1] 马挺[1] 李国强[1] 汪卫东[2] 李希明[2] 梁凤来[1] 蔡宝立[1] 刘如林[1] 

机构地区：[1]分子微生物学与技术教育部重点实验室天津市微生物功能基因组学重点实验室南开大学生命科学学院,天津300071 [2]中国石化胜利油田分公司采油工艺研究院,东营257000

出　　处：《微生物学通报》2008年第9期1348-1352,共5页Microbiology China

基　　金：国家自然科学基金资助项目(No.50674058);中国石化集团重点基础研究项目(No.P06071-3)

摘　　要：从胜利油田油水样中分离到一株能够在60℃高温条件下利用烃类产生生物表面活性剂的菌株芽孢杆菌(Bacillus sp.)A1。结果表明:A1的细胞表面具有很强的疏水性,这有助于菌体细胞对烃类的摄取。该菌株对石油烃具有良好的乳化作用,并可在20%的高盐环境和100℃高温条件下仍显示很高的乳化活性。同时,A1可明显改变油藏岩石表面的润湿性,使其亲水性显著增强。对油藏中的岩石模拟试片石英、灰岩和玻璃作用后的接触角均减小60%以上。油藏中岩石的润湿性能增强,水驱油时更易于剥落滞留在岩石表面上的油滴或油膜,从而提高石油采收率。A high temperature hydrocarbon-degrading strain, Bacillus sp. A1, was isolated from oil-water samples of ShengLi oil field in Shandong Province. The cell-surface hydrophobicity of A1, emulsification to hydrocarbons and change of the wetting property of rocks treated by A1 were investigated in this paper. Three methods were used to determine the cell-surface hydrophobicity, CAM （Contact Angle Measurement）, SAT （Salt Aggregation Test） and BATH （Bacterial adherence to hydrocarbon）. The results showed that the cell-Surface of A1 was strongly hydrophobic. The diesel oil can be well emulsified by A1, and the emulsion showed good tolerance to salinity, pH and temperature. The hydrophobicity of rocks surface decreased dramatically treated by A1 and the contact angles of quartz, limestone and glass fell more than 60%. If the hydrophilicity of the rocks is higher, the oil in the rocks could be recovered more easily.

关 键 词：烃降解菌A1 疏水性 乳化活性 岩石表面润湿性 

分 类 号：X172[环境科学与工程—环境科学]