散发性结直肠癌染色体7q21-22区杂合性缺失精细定位  

Refinement of Heterozygosity Loss on Chromosome 7q21-22 Region in Sporadic Colorectal Cancer

作　　者：许世峰[1] 徐延田[1] 彭志海[2] 

机构地区：[1]山东省立医院普外科,济南250021 [2]上海市第一人民医院普外科,上海200080

出　　处：《中国普外基础与临床杂志》2008年第9期637-640,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：国家自然科学基金资助项目(编号:30080016)~~

摘　　要：目的研究散发性结直肠癌7号染色体杂合性缺失,对7q21-22区精细定位,寻找新的结直肠癌抑癌基因。方法采用15对微卫星DNA标记7号染色体,在高频杂合缺失区另取5对微卫星标记对83例结直肠癌病例的肿瘤和正常组织进行PCR反应。PCR产物在ABIPrism377自动荧光测序仪进行电泳3h,以GeneScan3.1和Genotyper2.1软件进行基因分型。结果在7号染色体上发现1个高频杂合缺失区即7q21-22区。对该区再用5对微卫星标记引物行精细定位,界定了1个跨越D7S657、D7S646位点精细的高频杂合缺失区域。结论通过精细杂合缺失作图的研究,在7号染色体发现了1个跨越D7S657、D7S646位点的精细杂合缺失区,该区很可能存在1个或多个与结直肠癌相关的新的抑癌基因。Objective To refine the loss of heterozygosity （LOH） on chromosome 7q21-22 and identify the new tumor suppressor gene（s） in coloreetal tumorigenesis. Methods Fifteen polyrnorphie microsatellite markers were analyzed on chromosome 7 and another 5 markers were applied on chromosome 7q21-22 region in 83 cases of colorectal cancer and normal DNA by PCR. PCR products were elemrophoresed on an ABI Prism 377 DNA sequencer. GeneScan 3.1 and Genotyper 2. 1 software were used for LOH scanning and analysis. Results A distinct region of frequent allelic deletion was observed on chromosome, another ,5 polymorphic microsatellite markers were applied to 7q1 - 22 and the minimal region of frequent LOH was established on 7q21 -22 spanning the D7S657, D7S646 locus. Conclusion Through detailed deletion mapping studies, a critical and precise region spanning the D7S657, D7S646 locus is identified, which must contain one or more unknown tumor suppressor gene（s） on colorectal cancer.

关 键 词：散发性结直肠癌 杂合性缺失 精细定位 

分 类 号：R735.34[医药卫生—肿瘤]