胰腺癌BxPC3细胞中Src激酶对Notch-1活化的调节  

Tyrosine kinase Src mediates Notch-1 activation in BxPC3 pancreatic cancer cells

作　　者：杨小燕[1] 张玉祥[1] 王泽生[1] 

机构地区：[1]首都医科大学基础医学院生物化学与分子生物学系,北京100069

出　　处：《基础医学与临床》2008年第9期964-968,共5页Basic and Clinical Medicine

基　　金：北京市自然科学基金(7062007)

摘　　要：目的探讨在胰腺癌细胞BxPC3中,Src激酶对Notch-1活化的影响。方法用siRNA干扰的方法分别抑制Notch-1和c-Src的表达;加入Src激酶抑制剂PP2抑制Src激酶活性;MTT法检测细胞的生长;Western blot检测Notch-1蛋白活性形式NICD水平的变化。结果抑制Notch-1表达及抑制Src激酶活性可明显抑制BxPC3细胞生长;抑制Src激酶活性及抑制c-Src蛋白表达可下调Notch-1 NICD水平。结论Src激酶在胰腺癌细胞BxPC3中促进Notch-1的活化,促进BxPC3细胞的生长。Objective To observe the effect of tyrosine kinase Src on activating Notch-1 protein in pancreatic cancer cell line-BxPC3. Methods Notch-1 expression and c-Src expression were inhibited by siRNA interference, and the activation of Src was inhibited by its inhibitor PP2. The growth rate of BxPC3 ceils was measured by MTI＇. The change of Notch-1 NICD level and Src protein level was measured by Western blot. Results The growth of BxPC3 ceils decreased after inhibiting Notch-1 expression, as well as the ceils were treated with PP2. The level of Notch-1 NICD decreased both after inhibiting c-Src expression and after Src activation was inhibited by PP2. Condusion In BxPC3 cells, tyrosine kinase Sre promotes the level of Notch-1 NICD, which directly influences the cell growth.

关 键 词：胰腺癌 BXPC3 NOTCH-1 SRC PP2 

分 类 号：R735.9[医药卫生—肿瘤]