双液相系统中生物降解植物甾醇制备雄烯二酮工艺  

Preparing Androstenones by Microbial Degradation of Phytosterol in Biphasic System

作　　者：杨英[1] 姜绍通[1] 操丽丽[1] 李悦[1] 

机构地区：[1]合肥工业大学生物与食品工程学院

出　　处：《农业机械学报》2008年第9期79-82,86,共5页Transactions of the Chinese Society for Agricultural Machinery

基　　金：国家"十一五"科技支撑计划资助项目(项目编号:2006BAD05A12);安徽省自然科学基金资助项目(项目编号:070413265X)

摘　　要：在双液相系统中,研究植物甾醇生物降解制备雄烯二酮(AD)的工艺,并考察底物投料量以及添加表面活性剂、羟化酶抑制剂对降解的影响。结果表明:葵花油是分枝杆菌降解甾醇生成AD的最佳有机溶剂,底物投料量以及羟化酶抑制剂对转化有一定的影响。通过正交试验,确定了葵花油/水系统最优转化条件:油相浓度为20%,底物投料量为0.4%,添加剂为0.005%氯化汞,此工艺条件下,AD转化率高达83.4%。The technology of obtaining androstenones （AD） from phytosterol by microbial degradation in biphasic systems and the effects of substrate concentration, surfactants and 9α-hydroxylase inhibitor on hiotransformation were studied by experiment. The results show that sunflower oil-aqueous biphasic system is the most suitable for bioconversion, and the concentration of substrate and low-concentration of 9α-hydroxylase inhibitor can increase the rate of hiotransformation to a certain extent. Finally, the sunflower oil-aqueous biphasic system of fermentation is optimized by orthogonal experiment, and the optimal condition is as following： systems with the concentration of 20% sunflower oil, 0.4% substrate and 0. 005 % HgCl2. Under this condition the transformation rate of AD is 83.4 %.

关 键 词：雄烯二酮 双相系统 植物甾醇 生物降解 

分 类 号：TQ920.1[轻工技术与工程—发酵工程] X172[环境科学与工程—环境科学]