Deglycosylation altered the gating properties of rNav1.3:glycosylation/deglycosylation homeostasis probably complicates the functional regulation of voltage-gated sodium channel  

去糖基化改变rNav1.3门控性质:糖基化/去糖基化的动态平衡可能使电压门控钠通道的调控功能更具复杂性(英文)

作　　者：徐清[1] 程慧雯[1] 何慧琼[2] 刘志睿[1] 贺明[1] 杨宏天[1] 周智磊[1] 吉永华[1] 

机构地区：[1]上海大学生命科学学院,上海200444 [2]中国科学院上海生命科学研究院,上海200031

出　　处：《Neuroscience Bulletin》2008年第5期283-287,共5页神经科学通报（英文版）

基　　金：the National Basic Research Development Program of China (No. 2006CB500801).

摘　　要：Objective To examine the effect of deglycosylation on gating properties of rNav1.3. Methods rNav1.3 was expressed in Xenopus oocyte, with glycosylation inhibition by using tunicamycin. Two-electrode voltage clamp was employed to record the whole-cell sodium current and data were analyzed by Origin software. Those of glycosylated rNav1.3 were kept as control. Results Compared with glycosylated ones, the steady-state activation curve of deglycosylated rNav1.3 was positively shifted by about 10 mV, while inactivation curve was negatively shifted by about 8 mV. Conclusion Glycosylation altered the gating properties of rNav 1.3 and contributed to the functional diversity.目的观察糖基化对rNav1.3电压门控性质的影响。方法将rNav1.3在爪蟾卵母细胞中表达,衣霉素抑制其糖基化,双电极电压钳记录全细胞电流,Origin软件分析处理数据。对照组为未去糖基化的rNav1.3。结果与糖基化的rNav1.3相比,去糖基化的rNav1.3稳态激活曲线向去极化方向偏移约10mV,稳态失活曲线向超极化方向偏移约8mV。结论糖基化修饰改变rNav1.3的电压门控性质,异化其生理功能。

关 键 词：rNav1.3 voltage-gated sodium channel GLYCOSYLATION two-electrode voltage clamp Xenopus oocyte 

分 类 号：Q42[生物学—神经生物学]