野皂荚多糖胶酶法制备半乳甘露低聚糖的研究  

Study on production of galacto-mannan-oligosaccharides by β-mannanase hydrolyzing G.heterophylla gum

作　　者：张敏[1] 史劲松[2] 孙达峰[2] 张卫明[1] 

机构地区：[1]南京农业大学食品学院,江苏南京210095 [2]南京野生植物综合利用研究院,江苏南京210042

出　　处：《食品工业科技》2008年第9期57-59,62,共4页Science and Technology of Food Industry

基　　金："十一五"支撑计划课题(2006BAD06B05)

摘　　要：研究了β-甘露聚糖酶水解野皂荚多糖胶制备半乳甘露低聚糖的工艺条件以及水解产物中寡糖的组成,结果表明,反应时间和温度对酶解过程影响较大,而pH的影响相对较小。通过正交实验确定了酶法制备半乳甘露低聚糖的最佳工艺:底物浓度4.0%,加酶量700U/g,水解温度65℃,反应体系pH 6.5,水解时间8h。水解液平均聚合度为5.6,经TLC检测,水解产物主要为二糖以上的寡糖,HPLC分析表明,产物中半乳甘露低聚糖纯度达到74%。The optimum conditions of production of galacto-mannan-oligosaccharides were investigated using β-1,4-D-mannanse acted on G. heterophylla gum and analyzed the hydrolysis. The optimum condition was. G. heterophylla gum concentration 4.0 %, enzyme activity 700U/g, time 8.0h, temperature 65 ℃, pH 6.5, the yield was 26.8% under the best optical conditions. The primary factors of this reaction were time and temperature,while the least one was pH. The average polymeric degree of the hydrolysis was 5.6. By the TLC detection,the major was oligosaccharides above the disaccharide.

关 键 词：Β-甘露聚糖酶 野皂荚胶粉 半乳甘露低聚糖 薄层色谱 

分 类 号：TS201.23[轻工技术与工程—食品科学]