杂色曲霉素对小鼠小脑Purkinje细胞TNF-α和TGF-β_2 mRNA表达的影响  

Effects of sterigmatocystin on mRNA expressions of TNF-α and TGF-β_2 in Purkinje cells of mice

作　　者：郝庆卯[1] 姚荣芬[2] 张祥宏[3] 邢凌霄[3] 严霞[3] 夏建春[1] 许力军[1] 

机构地区：[1]石家庄卫生学校医学技术专业部,石家庄050200 [2]河北医科大学第四医院妇产科,石家庄050016 [3]河北医科大学病理学研究室,石家庄050017

出　　处：《神经解剖学杂志》2008年第5期514-517,共4页Chinese Journal of Neuroanatomy

基　　金：教育部科学技术研究重点项目(No205021);河北省自然科学基金(No301350);河北省卫生厅科研基金(No07373)资助项目

摘　　要：本研究观察了杂色曲霉素(sterigmatocystin,ST)对小鼠Purkinje细胞TNF-α和TGF-β2 mRNA表达的影响。给予BALB/c小鼠一次灌胃ST(3000μg/kg),分别于灌胃后0.5、1、2、4、8和16h处死动物,用原位杂交方法观察了Purkinje细胞TNF-α和TGF-β2 mRNA的表达。TNF-α和TGF-β2mRNA在对照组和处理组各时间点的Purkinje细胞均有阳性表达。但各处理组与对照组比较,TNF-α和TGF-β2 mRNA表达显著增加(P<0.01)。上述结果说明单次ST灌胃后TNF-α和TGF-β2 mRNA在Purkinje细胞表达明显增加,TNF-α和TGF-β2在ST对脑组织的损伤过程中起着重要的作用。To investigate the effects of sterigmatocystin （ST） on mRNA expressions of TNF-α and TGF-β2 in Purkinje cells of mice, BALB/c mice were treated intragastrically by garage with ST at 3000 μg/kg. The expressions of TNF-α and TGF-β2 mRNA in Purkinje cells of mice were detected by in situ hybridization 0.5, 1,2, 4, 8 and 16 h after oral administration, respectively. The results showed that TNF-α and TGF-β2 mRNA expressions could be found in some Purkinje cells at every time point in both control and treated groups. The positive rate and intensity of TNF-α and TGF-β2 mRNA expressions were all significantly higher than those in control group （ P 〈 0. 01 ）. The present results suggest that TNF-α and TGF-β2 mRNA expression were all significantly higher in Purkinje cells after oral ST. TNF-α and TGF-β2 might play a critical role in the injury of brain by ST.

关 键 词：杂色曲霉素 TNF-Α TGF-Β2 原位杂交 Purkinje细胞 小鼠 

分 类 号：R361.2[医药卫生—病理学]