陕西部分地区零售肉中沙门菌耐药的检测分析  

Detection and analysis of antibiotic resistance of salmonella from retail meats in some districts of Shaanxi province

作　　者：申进玲[1] 杨保伟[1] 只帅[1] 崔生辉[2] 席美丽[1] 杨鹏飞[3] 孟江洪[1] 

机构地区：[1]西北农林科技大学食品科学与工程学院,陕西杨凌712100 [2]北京中国药品生物制品检定所 [3]陕西杨凌西北农林科技大学理学院

出　　处：《中华预防医学杂志》2008年第10期758-761,共4页Chinese Journal of Preventive Medicine

摘　　要：目的对陕西省西安市和杨凌示范区超级市场及农贸市场零售肉中沙门菌的药敏性进行检测分析。方法采用美国临床实验室标准化委员会（National Committee of Clinical Laboratory Standard，NCCLS）推荐的琼脂稀释法，以大肠埃希菌ATCC25922和粪肠球菌ATCC29212为质控菌株，对193株沙门菌进行了7大类共14种抗生素的药敏性检测。结果沙门菌分离株对磺胺甲嗯唑的抗性最高（44．6％），其次为卡那霉素（40．9％）、四环素（37．8％）、阿莫西林（26．9％）、氨苄西林（25．4％）、庆大霉素（23．3％）和氯霉素（21．8％）等；对氟喹诺酮类抗生素的耐药率依次分别为环丙沙星22．3％、恩诺沙星21．8％、左氧氟沙星20．7％、加替沙星20．2％。55株（28．5％）沙门菌为多重耐药株，其中28株（14．5％）可耐至少13种抗生素，24株（12．4％）的耐药介于4到12种之间。结论陕西西安地区零售肉沙门菌多重耐药现象比较严重。Objective Salmonella isolates recovered from retail meats that were collected in supermarkets and free markets in Xi＇an and Yangling areas of Shaanxi province were studied to determine antibiotic susceptibility. Method Antimicrobial susceptibility to 14 antibiotics of 193 salmonella isolates were determined by using agar dilution method, which was recommended by National Committee of Clinical Laboratory Standard （NCCLS）, and E. coli ATCC25922 and E.faecalis ATCC29212 as standard control strains. Results The 44. 6% of the salmonella isolates were resistant to sulfamethoxazole, followed by resistance to kanamycin （ 40. 9% ）, tetracycline （ 37.8% ）, amoxicillin （ 26. 9% ）, ampicillin （ 25.4% ）, gentamicin （23.3%）and chloramphenicol （21.8%）.Some isolates also showed resistance to fluoroquinolones, the rates for ciprofloxacin, enrofloxacin, levofloxaein and gatifloxacin were 22. 3%, 21.8%, 20. 8% and 21.2%, respectively. 55 isolates （28.5%） were muhidrug resistant （MDR） strains, 28 of 193 isolates （ 14. 5% ） could resist at least 13 antibiotics, 24 isolates （ 12. 4% ） were resistant to from 4 to 12 antibiotics. Conclusion Salmonella isolates recovered from retail meats in Xi＇an district of Shaanxi province were seriously resistant to antimicrobials commonly used as human and veterinary medicine.

关 键 词：肉 沙门菌属 抗药性 细菌 食品微生物学 

分 类 号：R686[医药卫生—骨科学]