Osteogenic-related gene expression profiles of human dental follicle cells induced by dexamethasone  

作　　者：Zuo-lin JIN Yong-kuan ZHANG Hai-yan SUN Zhu LIN Ying-chun BI Yin-zhong DUAN Yin DING 

机构地区：[1]Department of Orthodontics, School of Stomatology, Fourth Military Medical University, Xi'an 710032, China

出　　处：《Acta Pharmacologica Sinica》2008年第9期1013-1020,共8页中国药理学报（英文版）

基　　金：Project supported by grants from the National Natural Science Foundation of China （No 30400510）. Zuo-lin JIN designed research; Yong-kuan ZHANG and Hai-yan SUN performed research; Ying-chun BI and Yin-zhong DUAN contributed new analytical reagents and tools; Yin DING analyzed data; Zuo-lin JIN and Zhu L1N wrote the paper.

摘　　要：Aim： Human dental follicle cells （hDFC） have the ability to differentiate into mineralized tissue-forming cells during root and periodontal development or osteogenic induction in vitro. The present study aimed to validate the osteogenic induction of hDFC by dexamethasone （DEX） and to explore the changes of related genes responsible for the osteogenic differentiation process. Methods： Passage-cultured hDFC were induced by DEX and analyzed for mineralization activity by morphological observation, alkaline phosphatase （ALP） activity, and alizarin red S staining. GEArray Q series human osteogenesis gene array was used to describe large-scale gene expression in treated hDFC compared to the control group. Quantitative real-time RT-PCR was performed to confirm the microarray data by analyzing the expression of 7 critical transcripts. Results： Osteogenic differentiation of hDFC was confirmed by morphological change, elevated ALP activity and calcified nodules. In 96 genes investigated through the microarray analysis, 20 genes were upregulated and 8 genes were downregulated more than 2-fold. The results of the real-time RT-PCR correlated with the microarray analysis. The expression of the transforming growth factor-β superfamily showed varying degrees of increase, and fibroblast growth factors exhibited a differential changing trend of expression. The expression of most types of collagen genes representative of extracellular matrixes increased under DEX treatment while small mothers against decapentaplegic 6 and 7 expressions significantly decreased. Conclusion： Our results demonstrated that hDFC displayed osteoblastic features in both phenotypic and genotypic traits induced by DEX in vitro.Aim： Human dental follicle cells （hDFC） have the ability to differentiate into mineralized tissue-forming cells during root and periodontal development or osteogenic induction in vitro. The present study aimed to validate the osteogenic induction of hDFC by dexamethasone （DEX） and to explore the changes of related genes responsible for the osteogenic differentiation process. Methods： Passage-cultured hDFC were induced by DEX and analyzed for mineralization activity by morphological observation, alkaline phosphatase （ALP） activity, and alizarin red S staining. GEArray Q series human osteogenesis gene array was used to describe large-scale gene expression in treated hDFC compared to the control group. Quantitative real-time RT-PCR was performed to confirm the microarray data by analyzing the expression of 7 critical transcripts. Results： Osteogenic differentiation of hDFC was confirmed by morphological change, elevated ALP activity and calcified nodules. In 96 genes investigated through the microarray analysis, 20 genes were upregulated and 8 genes were downregulated more than 2-fold. The results of the real-time RT-PCR correlated with the microarray analysis. The expression of the transforming growth factor-β superfamily showed varying degrees of increase, and fibroblast growth factors exhibited a differential changing trend of expression. The expression of most types of collagen genes representative of extracellular matrixes increased under DEX treatment while small mothers against decapentaplegic 6 and 7 expressions significantly decreased. Conclusion： Our results demonstrated that hDFC displayed osteoblastic features in both phenotypic and genotypic traits induced by DEX in vitro.

关 键 词：human dental follicle cell DEXAMETHASONE eDNA microarray DIFFERENTIATION 

分 类 号：R96[医药卫生—药理学]