中药桂皮醛体外抑菌和抗炎活性的研究  

Antibacterial Activities Against Oral Pathogen Standard Strains and Anti-inflammation of Chinese Medicine Cinnamicaldehyde in Vitro

作　　者：赵美林[1] 黄萍[1] 杨霞[2] 戚刚刚[2] 肖丽英[2] 肖晓容[2] 

机构地区：[1]四川大学华西口腔医院牙周科,四川成都610041 [2]口腔生物医学工程教育部重点实验室

出　　处：《广东牙病防治》2008年第10期441-443,共3页Journal of Dental Prevention and Treatment

基　　金：成都市科技攻关项目(07ggyb055sf-010)

摘　　要：目的检测桂皮醛的体外抗菌及抗炎活性。方法采用美国临床实验室标准化委员会推荐的液体稀释法将P.gingivali ATCC33277、A.viscosus ATCC15987、F.nucleatum ATCC10593、S.mutans ATCC25175等4种标准菌株配成1×108菌落形成单位/mL和1×106菌落形成单位/mL的细菌悬液,分别检测桂皮醛对其的抑菌浓度。通过大鼠双侧耳廓二甲苯致炎建立肿胀模型,进行桂皮醛对肿胀抑制的体外抗炎活性实验,观察比较大鼠双侧耳廓肿胀抑制厚度以及肿胀抑制率。结果①桂皮醛对P.gingivalis及F.nucleatum的最低抑菌浓度和最低杀菌浓度均为32μg·mL-1,对A.viscosus和S.mutans的抑菌浓度分别是64μg·mL-1和128μg·mL-1。②与对照组相比,24h、48h后桂皮醛对大鼠耳廓的肿胀抑制率分别为73.77%、68.42%。结论桂皮醛对口腔标准致病菌有抑菌活性,有体外抗炎活性。Objective To investigate antibacterial activities against main oral pathogen and anti-inflammation of Chinese Medicine Cinnamicaldehyde （CA） in vitro. Methods （1）the liquid dilution was used to detect the minimal inhibition concentration （MIC） and minimal bacteriocidal concentraction （MBC） of Cinnamicaldehyde; （2）Rat ear edema inhibition model was used to investigate the anti-inflammation effect of CA. Ten rats were divided randomly into 2 groups and self control was applied ： Group of cinnamaldehyde, group of physiological saline. The edema level of bilateral auricles induced by inunction of dimethylbenzene were measured either before and 1 hour, 24 hours, 48 hours after inunction. Resuits To P. gingivalis ATCC33277, A. viscosus ATCC15987, F. nucleatum ATCC10593, S. mutans ATCC25175, the MIC of Cinnamicaldehyde were respectively 32μg·mL^-1 , 32 μg·mL^-1 , 64 μg·mL^-1 , 128 μg·mL^-1. The edema of rat aureole was obviously inhibited by Cinnamicaldehyde. After 24 h and 48 h, the extent of edema were inhibited by 73.77% and 68.42% repectively. Conclusion Cinnamicaldehyde show obvious antibacterial activities against main oral pathogen and anti-inflammation ability in vitro.

关 键 词：桂皮醛 抑菌活性 抗炎活性 口腔致病菌 

分 类 号：R780.2[医药卫生—口腔医学]