丝氨酸—苏氨酸蛋白激酶2蛋白表达在低氧致大鼠肺动脉平滑肌细胞增殖中的作用  

Changes of Akt 2 Protein Expression in Cell Proliferation of Pulmonary Arterial Smooth Muscle Cells induced by Hypoxia in Rats

作　　者：易斌[1] 钱桂生[1] 白莉[1] 王关嵩[1] 

机构地区：[1]第三军医大学新桥医院全军呼吸研究所,重庆市400038

出　　处：《中国动脉硬化杂志》2008年第8期597-599,共3页Chinese Journal of Arteriosclerosis

基　　金：国家自然科学基金(30400200;30700347)资助;重庆市自然科学基金(2007)资助

摘　　要：目的通过观察低氧致大鼠肺动脉平滑肌细胞丝氨酸/苏氨酸蛋白激酶2蛋白表达水平变化与低氧致肺动脉平滑肌细胞增殖的关系,探讨丝氨酸/苏氨酸蛋白激酶2在低氧肺血管重建中的调控作用。方法组织块法培养肺动脉平滑肌细胞,采用蛋白印迹技术检测蛋白表达水平,采用甲基噻唑基四唑法、氚—胸腺嘧啶核苷掺入法检测肺动脉平滑肌细胞增殖。结果低氧刺激肺动脉平滑肌细胞不断增殖,常氧下氚—胸腺嘧啶核苷掺入检测值为0.372±0.059,随着低氧处理时间的延长氚—胸腺嘧啶核苷掺入检测值不断升高,其中低氧12 h达到0.703±0.100,与常氧组比较差异显著;常氧下甲基噻唑基四唑检测值为8374.39±545.31,随着低氧处理时间的延长,甲基噻唑基四唑检测值不断升高,低氧24 h达到11208.35±678.82,与常氧组比较差异显著。各组均检测出丝氨酸/苏氨酸蛋白激酶2蛋白的表达,图像定量分析显示各组蛋白表达与细胞增殖改变密切相关。结论丝氨酸/苏氨酸蛋白激酶2信号通路可能在低氧肺动脉高压中调节肺动脉平滑肌细胞增殖。Aim To observe the changes of serine/threonine kinase 2（Akt 2）protein expression in pulmonary arteriy smooth muscle cells （PASMC） induced by hypoxia in rats, and to investigate the value of Akt 2 signaling pathway in hypoxia pulmonary hypertension （HPH）. Methods The pure PASMC was cultured by tissue-sticking methods. Akt 2 protein expressions were determined by Westem blotting after PASMC were exposed to hypoxia for 2 h, 8 h, 12 h and 24 h respectively. The changes of PASMC proliferation were determined by M＇IT and 3H-TdR incorporated way. Results The value of 3H-TdR was 0. 372 ±0. 059 under norrnoxia. And accompanying the prolongation of hypoxia time, the value of 3H-Td-Pt kept heightening. It was 0.703 ± 0. 100 in 12 h and significantly different from normoxia group; The value of MTT was 8374.39 ± 545.31 under normoxia. And accompanying the prolongation of hypoxia time, the value of MTT kept heightening. It was 11208.35 V 678.82 in 24 h group and significantly different from nomloxia group. The protein of Akt 2 could be detected in all groups, and the expressive level of protein showed a possible relationship with PASMC prolferation. Conclusion The results suggest that Akt 2 signaling pathway may play an important role in the hypoxia pulmonary hypertension.

关 键 词：病理学与病理生理学 肺动脉平滑肌细胞 丝氨酸/苏氨酸蛋白激酶 低氧肺动脉高压 

分 类 号：R363[医药卫生—病理学]