耐铅、铜微生物的筛选及吸附性能研究  

Study on Screening of Tolerant-Lead & Copper Strains and Its Adsorption Capability

作　　者：曹小红[1] 杨亚静[1] 鲁梅芳[1] 王春玲[1] 廖振宇[1] 包菁[1] 

机构地区：[1]天津科技大学食品工程与生物技术学院天津市食品营养与安全重点实验室,天津300457

出　　处：《环境保护科学》2008年第5期15-18,共4页Environmental Protection Science

摘　　要：采用浓度梯度筛选的方法,从重金属污染土壤中分离筛选到两株耐受和吸附铅、铜的细菌G、Y。用单因素的方法得到G最适的培养条件为:T=30℃;pH=6.0;NaCl浓度为0.5%;Y的最适培养条件分别为:T=37℃;pH=8.5;NaCl浓度为0.5%。用原子吸收分光光度法测得,G、Y的冻干菌体对Pb2+、Cu2+的吸附量分别为:G铅126.42mg/g,G铜75mg/g,Y铅61.08 mg/g,Y铜45.86 mg/g。G湿菌体对铅、铜的吸附率分别为:G铅94.66%,G铜92.84%;Y湿菌体对铅、铜的吸附率分别为:Y铅99.74%,Y铜97.96%。经检测,其遗传性状稳定。可进一步开发为重金属吸附剂。Two strains capable of tolerating and adsorbing Lead and Copper, was isolated from heavy metals--contaminated soils through concentration gradient screening technique, named G ＆ Y. By using the single factor method, the optimum growth conditions were determined, they were： G ： T30℃, pH6. 0, NaCl concentration 0. 5 %, Y ： T37℃, pH8. 5, NaCl concentration 0. 5%. Measured and analyzed by Determination Method- Atomic Absorption Spectrophotometry , Pb2＋, Cu2＋ could be adsorbed by freeze dried strains , the adsorption capacity of them are GPb 126.42mg/g,GCu 75 mg/g , YPb 61.08 mg/g , YCu 45. 86 mg/g. Pb2＋ ＆Cu2＋ could also be adsorbed by waterish strains, the adsorption rate of G were ,GCu92. 84 %, GYb 94. 66 %, the adsorption rate of Y were ： Yc. 97. 96 %, YPb 99. 74%. They were measured stable. And can be de- veloped as sorbents for bioremediation of heavy metals.

关 键 词：耐铅 铜菌株 分离纯化 吸附性能 

分 类 号：TQ424.1[化学工程] TS251.1[轻工技术与工程—农产品加工及贮藏工程]