海带蛋白的提取和活性研究  

Isolation and Determination of Biological Activities of Proteins in Kelp Laminaria japonica

作　　者：李伟[1] 邬丽君[1] 肖妍君[1] 金桥[1] 佟长青[1] 赵前程[1] 胥亚夫[1] 汪秋宽[1] 

机构地区：[1]辽宁省水产品加工与贮藏重点开放实验室,大连水产学院食品工程系,辽宁大连116023

出　　处：《水产科学》2008年第10期530-532,共3页Fisheries Science

基　　金：农业部资助项目;辽宁省自然科学基金资助项目(2050885);辽宁省教育厅资助项目(05L086);农业部暨辽宁省省级高校海洋水产增养殖学与生物技术重点开放实验室开放课题资助项目(K2006-09);大连水产学院科研基金资助项目(017050)

摘　　要：采用5％NaOH、0．15mol／L NaCl、0．1mol／L磷酸盐缓冲液、0．15mol／L NaCl及0．06％纤维素酶和1％SDS溶液提取新鲜海带中的蛋白质，并采用Brrddford的方法测定提取的蛋白质含量。结果表明，采用5％NaOH＋0．06％纤维素酶浸泡，然后经超声波破碎，离心取上清液进行100％饱和度的（NH4）2SPO4沉淀，对所得沉淀透析后冻干所得海带蛋白质的含量较多。时间飞行质谱表明蛋白质分子量为872～7705m／z。抑菌试验表明海带蛋白对大肠杆菌、产气杆菌、金色葡萄球菌具有抑制作用。同时，以海带蛋白（5mg／kg）对高血压模型大鼠灌胃具有良好的降压作用。The proteins were isolated from kelp Laminaria japonica by 5% NaOH, 0.15 mol/L NaCl, 0.1 mol/ L PB, cellulase（0.06% ）and 1% SDS. The protein content of the kelp samples was determined by Brrddford＇s method. More proteins was isolated from the kelp by the process of immersion in 5% sodium hydroxide and cellulase （0.06%）, followed by ultrasonic fracture and addition of pulverized ammonium sulphate to the supernatant up to 100% saturation. MALDI-TOF showed that the proteins from the kelp had molecular weight of 872 - 7705 m/z. The antibiotic test showed that the proteins had antibacterial activity of Escherichia coli, Enterobacter aerogenes and Staphylococcus aureus, and better antihypertensive effects on renovascular hypertensive rats.

关 键 词：海带蛋白 飞行时间质谱 抑菌活性 降血压 

分 类 号：Q532[生物学—生物化学]