Dynamic Study of the Effect of Electroacupuncture on Dopamine in the Striatum for Rats with Cerebral Ischemia and Reperfusion  

作　　者：徐鸣曙 葛林宝 徐佳 

机构地区：[1]Shanghai Research Institute of Acupuncture and Meridian,Shanghai 200030,P.R.China [2]Shanghai Research Center of Acupuncture and Meridian,Shanghai 201203,P.R.China Shanghai Research Institute of Qigong,Shanghai 200030,P.R.China [3]Shanghai Yue-yang Integrated Medicine Hospital Affiliated to Shanghai University of TCM,Shanghai 220437,P.R.China

出　　处：《Journal of Acupuncture and Tuina Science》2008年第5期295-297,共3页针灸推拿医学（英文版）

基　　金：the Science and Technology Commission of Shanghai Municipality(014319364);the Science Foundation of Shanghai Municipal Commission of Science and Technology(05DZ19745,06DZ19732,064319053,07DZ19722,07DZ19733);the National Basic Research Program of China(973 Program,2005CB523306);Shanghai Leading Academic Discipline Project(B112 and T0302)

摘　　要：Object： To observe the dynamic changes of dopamine （DA） in the striatum with the process of duplicating cerebral ischemia and reperfusion （CIR） model on rats. And observe the effect of electroacupuncture （EA） on such changes. Methods： Using microdialysis sampling, high performance liquid chromatography （HPLC） detecting technology, dynamic sampling from experimental animals at multiple moments to observe the changes of dopamine in striatum with ischemia. And observe the effect of EA at Fengchi （GB 20） on such changes. Result： About the extracellular level of DA in normal group, sham-operation group and sham-operation ＋ EA group, no significant changes were observed during the measuring period. The extracellular level of DA peaked at 15-45 min after MCAO and 0-30 min （P〈0.05） after reperfusion. A rising trend was also observed following 120 min after reperfusion. After EA, the extracellular level of DA was significant lower than ischemia group following 90 min after reperfusion （P 〈0.05）. And there was no peak again. Conclusion： EA at Fenchi （GB 20） can regulate the disorder of DA. EA can improve the integrity neurological function. This may be one of the mechanisms of acupuncture treating the lesion of cerebral ischemia and reperfusion.目的:观察脑缺血再灌注模型大鼠纹状体内多巴胺的动态变化,以及电针对该变化的影响。方法:运用微透析采样技术、高效液相检测技术,在多个时段对实验动物进行动态采样,观察缺血所致纹状体内多巴胺的变化,及电针风池穴对该变化的影响。结果:正常组、假手术组、假手术+电针组在观测时段内,细胞外多巴胺的变化没有显著差异,胞外多巴胺的含量在脑缺血后15-45 min 及再灌注后0-30 min 出现两个峰值(P<0.05)。再灌注后120 min 也观测到一次上升的趋势。经电针治疗后,胞外多巴胺的含量于再灌注后90 min 明显低于缺血组(P<0.05),再灌注后也未出现峰值。结论:电针风池穴能够调节多巴胺含量的紊乱,能够改善整体的神经功能。这可能是针灸治疗脑缺血再灌注损伤的机制之一。

关 键 词：ELECTROACUPUNCTURE Chromatography  High Pressure Liquid MICRODIALYSIS Points  Fengchi （GB 20） DOPAMINE RATS 

分 类 号：R2-03[医药卫生—中医学]