细胞凋亡荧光染色法在检测TIL细胞毒效应中的应用  

A new approach for measurement of TIL cytotoxicity using apoptosis fluorescence staining assay

作　　者：钟晓松[1,2] 张希衡[1,2] 童善庆[1,2] 朱佑明[1,2] 胡宝瑜[1,2] 丁建青[1,2] 陆德源[1,2] 

机构地区：[1]上海第二医科大学微生物学教研室,200025 [2]上海第二医科大学肿瘤生物治疗实验室,200025

出　　处：《上海医学》1997年第12期705-708,共4页Shanghai Medical Journal

摘　　要：细胞毒细胞杀伤肿瘤细胞主要通过细胞凋亡机制。用传统的MTT法探讨了肿瘤浸润淋巴细胞TIL细胞杀伤活性最佳条件，同时采用凋亡荧光染色法（AFS）检测TIL的生物学效应。结果发现AFS法比MTT法更能准确地反映TIL对靶细胞的杀伤，杀伤率与效靶比呈良好的线性关系（r=0．89）；且该法简便、快速、敏感、重复性好，能同时观察活细胞、凋亡及坏死三种细胞的形态特征。The apoptotic cells may be induced by the cytotoxicity cells. The authors detected the cytotoxicity of TIL by using apoptosis fluorescence staining. A comparision between the results of AFS assay with the results of MTT assay showed signigicant difference. There was a high degree of over a wide range of E/T ratios and cytotoxicity percentage (the r vaule is 0. 89) in AFS assay, while that of MTT assay showed no linear relationship. We found that the AFS as- say was a simple, rapid, accurate, sensitive and reproducible method to detect cytotoxicity and avoided different affect- ing factors of the MTT method. In addition, this new approach can differetiate concurrently whether these cells belong to apoptosis, necrosis or live cells.

关 键 词：肿瘤浸润 淋巴细胞 AFS MTT 胃肿瘤 

分 类 号：R735.204[医药卫生—肿瘤]