偶氮染料生物降解机理的研究  

Study on Biodegradation Mechanism of Azo Dyes

作　　者：严滨[1] 周集体[2] 柳广飞[2] 黄国和[1] 傅海燕[1] 柴天[1] 金磊[1] 石谦[1] 

机构地区：[1]厦门理工学院环境工程系,福建厦门361024 [2]大连理工大学环境与生命学院,辽宁大连116023

出　　处：《环境科技》2008年第5期1-5,共5页Environmental Science and Technology

基　　金：教育部跨世纪人才基金(科技函1999-2)

摘　　要：运用紫外光谱、液相色谱与质谱研究了偶氮染料的降解过程。通过紫外光谱分析,以酸性红B为底物测定了偶氮还原酶的酶活。以甲基红为底物,分析了偶氮染料的降解机理。提出并验证了在偶氮染料还原过程中,存在一个加氢的中间体。UV spectrophotometer, HPLC and MS were used to analyze the pathway of degradation of azo dyes. The enzyme activity was defined as the amount of reduced dyes by per mg protein per minute via UV spectrophotometric analysis using Acid Red B as substrate. And the mechanism for azo dyes biodegradation was also analyzed using Methyl Red as substrate. The partially reduced intermediate was found and confirmed.

关 键 词：偶氮染料 生物降解 液相色谱 质谱 

分 类 号：X2[环境科学与工程—环境科学]