M_3受体激动剂对氯化钡诱发大鼠心律失常的保护作用  

Protective effects of M_3 receptor agonists on the arrhythmias of rats induced by barium chloride

作　　者：李丹露[1] 王丽艳[1] 陈立朋[1] 张秀菊[1] 刘艳[1] 杨宝峰[1,2] 

机构地区：[1]哈尔滨医科大学药学院药理学教研室 [2]黑龙江省生物医药重点实验室.省部共建国家重点实验室培训基地,黑龙江哈尔滨150086

出　　处：《中国药理学通报》2008年第10期1328-1330,共3页Chinese Pharmacological Bulletin

基　　金：国家重点基础研究发展计划(973计划)资助项目(No2004CCAO6700);黑龙江省青年科学技术专项资金(NoQC05080);黑龙江省博士后启动基金资助项目

摘　　要：目的观察M3受体激动剂胆碱和毛果芸香碱对氯化钡诱发Wistar大鼠心律失常的保护作用。方法舌下静脉注射氯化钡诱发Wistar大鼠心律失常,分别给予胆碱和毛果芸香碱以及M3受体特异阻断剂4-二苯乙酰氧-N-甲基哌啶甲碘化物(4-DAMP)干预,观察大鼠心律失常的发生和发展情况。结果氯化钡4mg·kg-1可诱发大鼠出现明显的双向性室性心律失常,胆碱10mg·kg-1和毛果芸香碱0.2mg·kg-1均能推迟双向性室性心律失常的出现,并使心律失常的持续时间缩短(P<0.01)、严重程度减轻(P<0.01)。胆碱和毛果芸香碱的上述作用又被M3受体阻断剂4-DAMP0.12μg·kg-1部分阻断(P<0.05)。结论胆碱和毛果芸香碱通过激动心肌M3受体,对氯化钡诱发Wistar大鼠心律失常的发生和发展具有保护作用。Aim To observe the protective effects of choline and pilocarpine, the M3 receptor agonists, on the arrhythmias of Wistar rats induced by barium chloride. Methods Barium chloride was used to induce the experimental arrhythmias of Wistar rats. Choline, pilocarpine, and 4-diphenylaeetoxy-N-methylpiperidinemethiodide （4-DAMP）,the selective antagonist of M3 receptor,were used to explore the effects of M3 receptor on the arrhythmias induced by barium chloride. The oecurence and the severity of arrhythmias were observed. Results Choline 10 mg · kg^-1 and pilocarpine 0. 2mg· kg^-1 inhibited shortened the duration decreased the score of the occurence of arrhythmias, of arrhythmias （P 〈0. 01 ） and Mest （severity of arrhythmias） （P 〈 0. 01 ）. However, the beneficial effects were reversed （P 〈0.05 ） by 4-DAMP 0. 12 μg · kg^-1 ,the selective antagonist of M3 receptor. Conclusion Both choline and pilocarpine have protective effects on the arrhythmias of Wistar rats induced by barium chloride by activating the myocardial M3 receptor.

关 键 词：M3受体激动剂 胆碱 毛果芸香碱 4-DAMP 氯化钡 心律失常 

分 类 号：R-332[医药卫生] R284.1