微透析装置连结高效液相色谱法分析果汁饮料中的儿茶素  

ANALYSIS OF CATECHINS IN FRUIT JUICES BY MICRO-DIALYSIS INSTRUMENT CONNECTED WITH HPLC

作　　者：干宁[1] 李春野 李天华[1] 王鲁雁[1] 徐伟民[1] 

机构地区：[1]新型功能材料及其制备科学国家重点实验室基地,宁波大学材料科学与化工学院,宁波315211 [2]宁波市疾病预防与控制中心,宁波315000

出　　处：《营养学报》2008年第5期515-519,共5页Acta Nutrimenta Sinica

基　　金：浙江省自然科学基金(No.Y106725);浙江省科技攻关项目(No.2006C31040);浙江省教育厅基金(No.20061697);宁波市自然科学基金(No.2006A610048)资助项目

摘　　要：目的以微透析装置连结高效液相色谱(MD-HPLC)分离检测果汁中的七种儿茶素:儿茶素没食子酸酯(CG)、表儿茶素(EC)、表儿茶素没食子酸酯(ECG)、没食子儿茶素(GC)、没食子儿茶素没食子酸酯(GCG)、表没食子儿茶素(EGC)、表没食子儿茶素没食子酸酯(EGCG)的含量。方法样品直接注入微透析装置收集20分钟透析液后,通过HPLC分离检测。最佳化色谱条件为:使用C8色谱柱,流动相使用甲酸/甲醇/乙腈系统作梯度洗脱,流速:1.0ml/min。最佳微透析条件,流速:1.06μl/min,透析液收集时间:20min,灌流液:pH=4的乙腈-水(2:3)溶液。结果在最佳条件下,七种儿茶素单体在0.5～50mg/L内与峰面积值成正比,检测下限在0.01～0.1mg/L之间,加标回收率为92.5%-108.4%,RSD≤2.67%。结论本方法快速准确,可用于市售果汁中儿茶素含量的分离检测。Objective To analyze 7 catechins, namely catechin-3-O-gallate （CG）, epicatechin （EC）, epicatechin-3-O-gallate （ECG）, gallocatechin（GC）, gallocatechin-3-O-gallate（GCG）, epigallocatechin （EGC）, epigallocatechin-3-O-gallate （EGCG） in fruit juice by micro-dialysis connected with HPLC. Methods The samples were collected after microdialysis for 20 min then separated by HPLC. The optimal conditions of the micro-dialysis sampling were 40% acetonitrile as perfusate with the flow rate of 1.06 μl/min and 20 min collection time. And the optimal conditions of HPLC analysis were C8 reversed phase column with the mobile phase of acetonitrile, menthol and formic acid buffer with flow rate of 1 ml/min. Results By the optimal conditions, the calibration curve of catechins＇ peak areas were linear over the range of 0.5--50 mg/L, recoveries of micro-dialysis were 92.50% --108.36% with RSD≤2.67%. And the detection limits were 0.01 --0. lmg/L for the system of micro-dialysis connected with HPLC. Conclusion The method is fast, simple and reproducible and is suitable for examining catechins in fruit juices.

关 键 词：儿茶素 HPLC 微透析 果汁 

分 类 号：TS275.5[轻工技术与工程—农产品加工及贮藏工程]