诱导条件对重组人锰超氧化物歧化酶发酵的影响  

Effect of Inducement on the Expression of Recombinant Human Manganese Superoxide Dismutase

作　　者：陈车生[1] 吴乾一[2] 袁勤生[2] 吴梧桐[1] 

机构地区：[1]中国药科大学生命科学与技术学院,南京210009 [2]华东理工大学生物反应器工程国家重点实验室,上海200237

出　　处：《华东理工大学学报（自然科学版）》2008年第5期646-649,708,共5页Journal of East China University of Science and Technology

摘　　要：分别研究了3种诱导剂和Mn2+浓度对重组人锰超氧化物歧化酶(rhMn-SOD)发酵表达的影响,并进一步优化了乳糖诱导rhMn-SOD表达的条件。结果表明:异丙基--βD-硫代半乳糖苷(IPTG)和乳糖诱导能够获得类似的表达效果,诱导的同时加入2 mmol/L的Mn2+能够明显提高rhMn-SOD的表达。进一步的实验结果表明:乳糖诱导的最佳浓度为7 mmol/L,最佳时机为接种后6 h,A600为2.1,诱导表达6 h rhMn-SOD活性和比活达到最高。The effects of three kinds of inducers and different concentrations of Mn^2＋ on the expression of recombinant human manganese superoxide dismutase （rhMn-SOD） were studied. The inducement conditions of lactose on the expression of SOD were further optimized. Results showed that IPTG and lactose had similar effects on the expression of rhMn SOD. Adding 2 mmol/L of Mn^2＋ at the time of inducement improved the expression of rhMn-SOD. Further studies indicated that the suitable inducement conditions were addition of 7 mmol/L lactose at 6 h after inoculum, and the cell optical density A600 could reach 2.1.

关 键 词：重组人锰超氧化物歧化酶 诱导 发酵 大肠杆菌 乳糖 

分 类 号：Q786[生物学—分子生物学]