P_(53)基因突变与宫颈癌关系的研究  

The study of relationship between mutation of P_(53) gene and cervical carcinoma

作　　者：李文惠[1] 陈卫[1] 

机构地区：[1]兰州医学院生化教研室

出　　处：《兰州医学院学报》1997年第4期1-2,共2页Journal of Lanzhou Medical College

基　　金：甘肃省自然科学基金

摘　　要：目的：通过对宫颈癌Ｐ＿（５３）基因７—８外显子突变的研究，探讨该基因的突变与宫颈癌的发生、发展之间的关系。方法：本文运用聚合酶链式反应（ＰＣＲ）方法，对宫颈癌组织Ｐ＿（５３）基因的７—８外显子是否突变进行了试验，并与非肿瘤的慢性宫颈炎（宫颈糜烂、宫颈息肉）组织作了对照。结果：１０例宫颈癌标本中有７例出现了Ｐ＿（５３）７—８外显子的突变，阳性率高；而８例作为对照的宫颈炎标本仅有１例出现可疑的阳性结果。结论：试验结果说明，宫颈癌和宫颈炎相比，前者Ｐ＿（５３）７—８外显子的突变率明显高于后者，此项研究对宫颈癌的临床诊断和判断预后有一定的作用。Objective:Thr relationship beween mutation of P53 gene and occurrence, development cervical carcinoma has been studied on the basis of researching P53 7-8exon mutation.Methods:The paper utilized ploymerase chain reaction（PCR）method，determmed mutation rate of P53 gene 7-8 exon for the tissue of cervical caIcinoma and compared withchronic cervicitis(noncancer).Results:The 7 cases out of the 10 cases of cervical carcinoma showed posititre.Only one outof the 8 cases of chronic cervicitis showed questionable peitive result.Conchusions:The results explessed that mutation rateof P53 7-8 exon of cervical careinoma was obvious higher than chronic cervicitis.The study plays an important role in clini-cal diagnose and prognosis for cervical carcinoma.

关 键 词：P53基因 外显子 宫颈癌 肿瘤发生 

分 类 号：R737.330.2[医药卫生—肿瘤]