利用甜高粱秸秆汁发酵生产丁醇、丙酮  

Production of acetone and butanol by fermentation of sweet sorghum stalk juice

作　　者：程意峰[1] 李世杰[1] 黄金鹏[2] 张茜茜[1] 汪秀芳[3] 

机构地区：[1]工业微生物湖北省重点实验室,湖北工业大学生物工程学院,武汉430068 [2]湖北省农科院粮食作物研究所,武汉430064 [3]中国科学院武汉植物园,武汉430074

出　　处：《农业工程学报》2008年第10期177-180,共4页Transactions of the Chinese Society of Agricultural Engineering

基　　金：工业微生物湖北省重点实验室开放基金([2004]4)

摘　　要：该试验以甜高粱秸秆汁作为生产丙酮、丁醇的发酵原料,从5种丙酮-丁醇菌中选出能够利用甜高粱秸秆汁且丁醇产量高的Bacillus acetobutylicum Bd3菌作为试验菌株,并对该菌株的发酵条件进行优化,得到的优化条件为:糖度为10oBrix的甜高粱秸秆汁,玉米浆含量5 g/L,接种量6%(v/v),(NH4)2SO4 5 g/L,KH2PO4 0.4 g/L,CaCO3 6 g/L,温度32℃,pH6.8,丁醇产量达到10.29 g/L。Sweet sorghum stalk juice was used as raw material for producing acetone and butanol by fermentation. Bacillus acetobutylicum Bd3 was selected from five different acetone-butanol producing strains, as experimental bacterium which produced more butanol than the four others. And the conditions of acetone-butanol fermentation by Bd3 were optimized： sweet sorghum stalk juice10° Brix, corn-steep 5 g/L, inoculation 6%（V/V）, （NH4）2SO4 5 g/L, KHEPO4 0.4 g/L, CaCO3 6 g/L, temperture 32℃, pH6.8, the output of butanol achieved 10.29 g/L.

关 键 词：发酵 丙酮 丁醇 甜高粱秸秆汁 

分 类 号：TQ920.6[轻工技术与工程—发酵工程]