Neuregulin-1温度依赖性抑制小鼠海马脑片CA1区的长时程增强  

Neuregulin-1 temperature-dependently inhibits the long-term potentiation in the CA1 region in mouse hippocampal slices

作　　者：陈永君[1] 张猛[1] 王璞[1] 朱心红[1] 高天明[1] 

机构地区：[1]南方医科大学神经生物学教研室,广东广州510515

出　　处：《南方医科大学学报》2008年第10期1771-1774,1778,共5页Journal of Southern Medical University

基　　金：国家自然科学基金委员会-广东省人民政府自然科学联合基金(U0632007)

摘　　要：目的研究不同温度条件下Neuregulin-1(NRG1)对小鼠海马脑片CA1区突触传递和突触可塑性的影响。方法制作成年小鼠离体海马脑片标本,采用细胞外微电极记录技术,记录小鼠海马脑片CA1区Schaffer侧枝诱发场兴奋性突触后电位(fEPSP),以及施高频强直刺激(HFS)诱导长时程增强(LTP)。分别在室温(26±1)℃和生理温度(32±1)℃条件下,观察NRG1对fEPSP和LTP的影响。结果(1)无论在室温或生理温度条件下,灌流NRG1前后fEPSP斜率的平均值无明显变化(P>0.05)。(2)室温灌流NRG1组与室温正常对照组相比,强直刺激后fEPSP斜率的平均值无明显变化(P>0.05);与生理温度正常对照组相比,生理温度灌流NRG1组强直刺激后fEPSP斜率平均值降低有显著性意义(P<0.01)。结论NRG1温度依赖性抑制小鼠海马脑片CA1区的长时程增强。Objective To investigate the effect of neurogulin-1 （NRG1） on the transmission and plasticity of CA1 synapses in mouse hippocampal slices at different temperatures. Methods Under room temperature （26±1℃） or physiologcial temperature （32±1℃）, field excitatory postsynaptic potential （fEPSP） evoked by extracellular microelectrode recording technique was recorded in the CA1 region in adult mouse hippocampal slices, and the long-term potentiation （LTP） was induced by high frequency stimulation （HFS）. The effects of NRG1 on the baseline tEPSP and induction of LTP in CA1 region were observed. Results No significant variation in the slope of fEPSP relative to the baseline fEPSP was observed after perfusion with NRG1 at room temperature or physiological temperature （P〉0.05）. After HFS at the room temperature, the mean slope of fEPSP in the slices perfused with NRG1 was similar to that of the control group （~0.05）, but HFS at the physiological temperature resulted in significant reduction in the mean slope offEPSP in the slices perfused with NRG1 （P〈0.01）. Conclusion NRG1 may temperature-dependently inhibit the induction of LTP in the CA1 region in mouse hippocampal slices.

关 键 词：NEUREGULIN-1 长时程增强 小鼠 海马脑片 

分 类 号：R749.3[医药卫生—神经病学与精神病学]