间期荧光原位杂交技术检测慢性淋巴细胞白血病12三体和11q23缺失  

Detection of 11q23 deletion and trisomy 12 in chronic lymphocytic leukemia by interphase fluorescence in situ hybridization

作　　者：程月新[1] 陈明芳[1] 徐卫[2] 陈天荣[1] 李丽[2] 沈其根[3] 李建勇[2] 

机构地区：[1]南通大学第四附属医院血液科,江苏盐城224006 [2]南京医科大学第一附属医院血液科 [3]盐城纺织职业技术学院卫生所

出　　处：《中国医师杂志》2008年第10期1297-1299,共3页Journal of Chinese Physician

基　　金：江苏省医学领军人才卫生部科研基金资助项目（WKJ2005-2-025）;江苏省盐城市医学重点人才项目资助项目（YC2007013）;江苏省盐城市科技计划项目资助项目（YK007129）.

摘　　要：目的研究慢性淋巴细胞性白血病（CLL）12三体（＋12）和11q23缺失[del（11q23）]的异常情况。方法分别应用12号着丝粒探针和位于11q23的序列特异性DNA探针ATM，对30例初发的CLL患者进行间期荧光原位杂交（I-FISH）检测，并和常规细胞遗传学检测结果进行比较。结果30例CLL患者中，常规细胞遗传学检测出4例（13．3％）有染色体异常，I-FISH检测出8例（26．7％）有染色体异常，其中5例＋12，3例del（11q23）。结论FISH技术是检测CLL患者染色体基因组异常的有效手段，＋12，11q-在B—CLL的预后意义有待进一步探讨。Objective To investigate the incidence of trisomy 12 （ ＋ 12） and 1 lq23 deletion [ del（ 11 q23） ] in chronic lymphocytic leukemia （CLL）. Methods Fluorescein labeled DNA probe 12 and sequence specific probe ATM for 11q23 were used to perform interphase fluorescence in situ hybridization （I-FISH） assays in 30 patients with CLL. The results were compared with that of conventional cytogenetic （CC） examination. Results With CC examination , only 4 cases（ 13.3% ） were found to have chromosomal abnormalities, whereas with I-FISH assay ,8 cases （26. 7% ） were found to have genomic aberrations, including trisomy 12 in 5 cases , deletion of 11q23 in 3 cases. Conclusion I-FISH is a useful method for detection of genomic aberration in CLL, the significance of trisomy 12 and del （ 11 q23） in predicting the prognosis of B-CLL need to be investigated further.

关 键 词：白血病 淋巴细胞 慢性/遗传孳 染色体缺失 原位杂交 荧光 

分 类 号：R733[医药卫生—肿瘤]