航天诱变啤酒酵母菌株的复壮与筛选  

Rejuvenation and screening of space mutation Saccharomyces cerevisiae strains

作　　者：马旭光[1] 张宗舟[1] 

机构地区：[1]天水师范学院生命科学与化学学院,甘肃天水741001

出　　处：《中国酿造》2008年第11期72-75,共4页China Brewing

基　　金：2007年天水师范学院院列重点项目(TSA0704);国家科技支撑计划项目"西北内陆灌区农田循环生产技术集成研究与示范"(2007BAD89B17)

摘　　要：对经航天诱变的啤酒酵母菌进行复壮,并对复壮后的酵母菌株从细胞大小、菌落形态、发酵力、增殖情况以及死灭温度等几方面进行了筛选。试验结果表明,诱变后菌株的发酵力、生长速度均优于出发菌株,死灭温度基本相同。并依此筛选出了1株各种生理性状都较好的诱变菌株,其适宜的生长培养基为麦芽汁培养基,适宜的培养时间为24h。The space mutated strains Saccharomyces cerevisiae were rejuvenated and screened according to cell size, colony morphology, fermentation power, growth rate and the lethal temperature. The results showed that the fermentation power and growth rate of mutated strains were improved compared to those of original ones although their lethal temperature were not significantly different. Based on these results, a mutated strain was screened which had better physiological characters. Its optimal cultivation medium was malt extract medium and cultivation time was 24 h.

关 键 词：航天诱变 啤酒酵母 复壮 筛选 

分 类 号：Q93-31[生物学—微生物学]