马齿苋总黄酮酶解提取工艺的优化  

Opimization of Cellulase-assisted Ethanol Extraction Technology of Total Flavonoids from Portulaca oleracea L.

作　　者：姜宁[1] 刘晓鹏[1,2] 王东辉[2] 向东山[1] 雷红灵[1] 翟琨[1] 

机构地区：[1]生物资源保护与利用湖北省重点实验室,湖北恩施445000 [2]湖北民族学院生物科学与技术学院,湖北恩施445000

出　　处：《食品科学》2008年第10期323-326,共4页Food Science

基　　金：湖北民族学院重点建设学科项目

摘　　要：目的:研究纤维素酶酶解辅助提取马齿苋总黄酮的的最佳工艺。方法:通过正交试验研究马齿苋总黄酮的乙醇浸提提取工艺;通过四元二次回归正交试验进行纤维素酶酶解辅助提取马齿苋总黄酮的研究。结果:乙醇浸提马齿苋总黄酮的最佳方法是:料液比1:30,浸提时间60min、水浴温度60℃,乙醇浓度60%。酶解辅助提取马齿苋总黄酮的最佳工艺为:酶浓度1.9‰、pH7.0、酶解温度47℃、酶解时间0.7h。结论:得到了纤维素酶总黄酮提取马齿苋总黄酮的最佳工艺,酶解辅助提取法显著优于乙醇浸提法。Objective： To optimize ethanol extraction technology of total flavonoids from Portulaca oleracea L.. Methods： The ethanol extraction technology of total flavonoids from Portulaca oleracea L. was optimized by orthogonal test, and the cellulase hydrolysis conditions were optimized by quadratic regression model in four variables-orthogonal test results. Results： The optimal conditions of ethanol extraction technology are as follows： material-liquid ratio 1：30, extraction temperature 60 ℃, time 60 min, and ethanol concentration 60%. The optimal cellulase hydrolysis conditions for extracting the total flavonoids from Portulaca oleracea L. with ethanol are as follows： cellulase amount 1.9‰, hydrolysis temperature 47 ℃, pH 7.0, and time 0.7 h. Conclusion： The cellulase-assisted method is much better than the ethanol extraction method.

关 键 词：马齿苋 总黄酮 酶解辅助提取 

分 类 号：TS201.1[轻工技术与工程—食品科学]