RP-HPLC法优化笃斯越橘花青素提取、纯化技术研究  

Study on Optimization of Extraction and Purification of Anthocyanidins from Vaccinium uliginosum L. by RP-HPLC

作　　者：董怡[1] 林松毅[1] 刘艳丰[1] 李明瑾[1] 张兴茂[1] 刘璇[1] 刘静波[1] 

机构地区：[1]吉林大学军需科技学院营养与功能食品研究室,吉林长春130062

出　　处：《食品科学》2008年第10期349-352,共4页Food Science

基　　金：2007年度"大学生创新实验计划"国家级资助项目(2007A83050)

摘　　要：通过对笃斯越橘花青素进行RP-HPLC分析,首先得到花青素提取率随盐酸浓度变化的规律,并得出盐酸-乙醇法提取花青素的最佳盐酸浓度为1.5mol/L。分别采用AB-8大孔树脂、聚酰胺树脂及AB-8大孔树脂与聚酰胺树脂联用对提取液进行了初步纯化,得到了4种纯化花青素样品,经过RP-HPLC法检测分析获得了4种纯化样品的花青素的纯度分别为13.36397%、36.95687%、13.21466%、8.559135%,可知采用聚酰胺树脂对笃斯越橘花青素的纯化效果明显优于AB-8树脂,纯度可提高23.5%。Depending on the detection of RP-HPLC, the change trend of the extraction rate of anthocyanins from Vaccinium uliginosum L. with the hydrochloric acid concentration was investigated. The best hydrochloric acid concentration is determined as 1.5 mol/L. The Vaccinium uliginosum L.extracts were purified by AB-8 macroporous resin, polyamide resin （eluted with 80% or 40% ethanol solution） and combination of the two resins, respectively, and four anthocyanins samples were obtained and then detected by RP-HPLC. The results showed that the anthocyanins purity of the four samples are 13.36397%, 36.95687%, 13.21466% and 8.559135%, respectively. According to the above results, the polyamide resin is apparently better than AB-8 macroporous resin in the purification of anthocyanins, and the anthocyanins purity is increased by 23.5%.

关 键 词：RP—HPLC 笃斯越橘 花青素 

分 类 号：O658[理学—分析化学]