中国马传染性贫血病毒DLV株前病毒gp90基因在马体内的变异分析  

Variation Analyses of gp90 Gene of Proviral DNA of Chinese Equine infectious anemia virus Donkey Leukocyte Attenuated Strain

作　　者：杨彬[1] 王雪峰[1] 周建华[2] 王凤龙[1] 

机构地区：[1]内蒙古农业大学动物科学与医学学院．内蒙古呼和浩特010018 [2]中国农业科学院哈尔滨兽医研究所兽医生物重点实验室大动物病研究室,黑龙江哈尔滨150001

出　　处：《动物医学进展》2008年第11期20-24,共5页Progress In Veterinary Medicine

基　　金：国家自然基金项目(30771994);哈尔滨市科技攻关计划项目(2006AA3AS040)

摘　　要：分别以EIAV辽宁马强毒株(EIAVliao)前病毒DNA和马传染性贫血驴白细胞弱毒疫苗株(EIAVDLV)前病毒DNA为模板,从免疫接种后不同时期马血清中利用nested-PCR技术,扩增了约1.4kb的gp90基因。将其克隆后进行了测序,测序结果表明,免疫EIAVDLV后的第1时期至第5时期,与EIAV-liao比较核苷酸序列平均差异率分别为3.2956%、3.1456%、3.36%、3.1856%和3.6456%。EIAVliao有20个N-连接糖基化位点,EIAVDLV平均是17.2个,其中有11个N-连接糖基化位点是高度保守的。Using nested-polymerase chain reaction technique, the gp90 gene of provious DNA from EIAV Liaoning strain （EIAVliao） and EIAV donkey leukocyte attenuated strain（EIAVDLv）was amplified from different episode of plasma after vaccination, and then cloned and sequenced. The sequenced result showed that from the first to the fifth episode post vaccination, average diversities of nucleotide sequence were 3. 295 6%,3. 145 6%,3.36%,3. 185 6%,3. 645 6%,respectively,compared to EIAVliao. EIAVliao gp90 has 20 potential N-linked glycosylation sites, EIAVDLV averagely has 17.2, of which has 11 highly conservative N-linked glycosylation sites.

关 键 词：马传染性贫血弱毒疫苗株 GP90基因 N-连接糖基化 序列分析 突变 

分 类 号：S852.659.3[农业科学—基础兽医学]