Polycomb家族基因Nspc1在斑马鱼早期发育的表达  

Expression Pattern of Polycomb Gene Nspc1 at the Early Developmental Stage in Zebrafish

作　　者：吴旭东[1] 张敏[2] 龚燕华[1] 强伯勤[1] 袁建刚[1] 孟安明[2] 彭小忠[1] 

机构地区：[1]中国医学科学院北京协和医学院基础医学研究所医学分子生物学国家重点实验室,北京100005 [2]清华大学生物科学与技术系分子发育生物学实验室,北京100084

出　　处：《中国医学科学院学报》2008年第5期550-553,共4页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金(30600166,30421003);国家重点基础研究发展计划项目(973计划) (2005CB522507);国家高技术研究发展计划项目(863项目) (2006AA02Z137)~~

摘　　要：目的探讨Polycomb家族基因Nspc1在模式生物斑马鱼发育早期的表达模式。方法根据GenBank中斑马鱼Nspc1的cDNA序列设计Nspc1原位杂交探针片断,并制备地高辛标记的探针。收取单细胞期、双细胞期、胚芽期及不同时间点的体节期斑马鱼胚胎,用制备好的探针作原位杂交,显微镜下拍照,获得信号适当的杂交结果。结果在斑马鱼体节期前,Nspc1在全身表达;从体节期开始,其表达逐渐表现出特异性,主要在头部的神经系统中表达。结论Nspc1在模式生物斑马鱼的早期发育、尤其是神经系统发育的过程中可能扮演重要角色。Objective To study the expression pattern of Polycomb gene Nspcl at the early develop- mental stage in zebrafish. Methods In situ hybridization probe for Nspcl was designed according to the Gen- Bank information. Collecting zebrafish embryos at different stages including one cell stage, two-cell stage, bud stage, and somites stage, we hybridized them with the prepared probe. Then the hybridization signals at different intervals were observed and photographed at the right early stage. Its expression specificity began at the somites time. Results Nspcl was expressed globally at the stage, mainly in the nervous system of the head.Conclusion Nspcl may play essential roles in the early stage development of zebrafish, especially in the nervous system.

关 键 词：POLYCOMB NSPC1 斑马鱼 神经系统发育 

分 类 号：Q344.13[生物学—遗传学]