苦丁茶水提物体外抗柯萨奇病毒B3活性的实验研究  

Study on anti-coxsackie virus effect of aqueous extracts from llex kudmcha c.j.tseng in vitro

作　　者：王亚峰[1] 张美英[1] 郝静[1] 王小燕[1] 钱垂文[1] 王一飞[1] 张颖君[2] 杨崇仁[2] Kaio Kitazato Nobuyuki Kobayash 

机构地区：[1]暨南大学生物医药研究开发基地,广州510632 [2]中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室,昆明650204 [3]日本国立长崎大学药学部感染分子研究室,长崎852-8521

出　　处：《中华中医药杂志》2008年第11期976-978,共3页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金;广东省人民政府自然科学联合基金(No.U0632010)

摘　　要：目的:探讨苦丁茶水提物体外抗柯萨奇病毒B3(CVB3)的作用及机制的研究。方法:采用组织细胞培养法,通过观察细胞病变(CPE)以及四甲基偶氮唑蓝(MTT)法检测细胞活性、测定病毒抑制率,以确定苦丁茶水提物体外抗病毒活性,并采用预防、治疗和同时用药干预的方法初步探讨其抗病毒作用机制。结果:苦丁茶水提物有明显的体外抗CVB3活性,对HeLa细胞的半数中毒浓度(TC50)为45700.3μg/ml,半数抑制浓度(IC50)为119.18μg/ml,治疗指数(TI)为383.46。结论:苦丁茶水提物在体外具有明显的抑制CVB3作用。Objective： To investigate the anti-coxsackie virus B3 effect of aqueous extracts from llex kudmcha c. j. tseng in vitro and their antiviral mechanism. Methods： The antiviral effects of the aqueous extracts from llex kudmcha c. j. tseng on CVB3 and their cytotoxicity on HeLa cells were studied by CPE and MTT methods. Antiviral mechanism of the aqueous extracts was also investigated by three different ways. Results： The aqueous extracts has a significant anti-CVB3 effect at cell level in vitro, showing TCs0 values of 45 700.3 μg/ml, IC50 values of 119.18 μg/ml, and TI of 383.46. Conclusion： The aqueous extracts from llex kudmcha c. j. tseng had a significant anti-CVB3 effect in vitro.

关 键 词：柯萨奇病毒B3 苦丁茶 细胞病变 四甲基偶氮唑蓝 

分 类 号：R285[医药卫生—中药学]