双水相萃取法提取虫草发酵液中甘露醇的研究  

Study on the extraction of mannitol from C. nigrella fermented broth by double aqueous phase system

作　　者：陈毅坚[1] 那吉[1] 达爱斯[1] 董学畅[1] 

机构地区：[1]云南民族大学化学与生物技术学院,昆明650031

出　　处：《食品科技》2008年第11期215-218,共4页Food Science and Technology

摘　　要：探索采用正丙醇/(NH4)2SO4双水相系统从拟黑虫草发酵液中提取甘露醇的方法。实验研究了正丙醇、(NH4)2SO4、溶液浓度和pH值对甘露醇分配系数、萃取率的影响,确定提取甘露醇的最佳条件,即正丙醇溶液浓度为20%、(NH4)2SO4溶液浓度为25%、pH7.4时分配系数为0.028,甘露醇萃取率最高为73.79%。This paper explored the method in which mannitol was extracted and purified by n-propyl alcohoV （NH4）2SO4 double aqueous phase system. The effects of n-propyl alcohol, （NH4）2SO4 solution concentration and pH value on distribution coefficient, extractive rate and phase separation time were studied. The optional conditions for mannitol extraction were determined. When the concentration of n-propyl alcohol solution was 20% and （NH4）2SO4 solution was 25%, pH value was 7.4, the smallest distribution coefficient and highest extractive rate were obtained as 0.028 and 73.79% respectively.

关 键 词：拟黑虫草发酵液 甘露醇 正丙醇/(NH4)2SO4 双水相 

分 类 号：TS201.2[轻工技术与工程—食品科学]