人工合成小麦中HMW-GS 6＋8和1.5＋10的品质效应  

Effect of HMW-GS 6+8 and 1.5+10 on Wheat Quality Traits in Synthetic Hexaploid Wheat

作　　者：汤永禄[1,2] 杨武云[2] 田纪春[3] 李俊[2] 陈放[1] 

机构地区：[1]四川大学生命科学学院,成都610081 [2]四川省农业科学院作物研究所,成都610066 [3]山东农业大学农学院,山东泰安250100

出　　处：《中国农业科学》2008年第11期3465-3476,共12页Scientia Agricultura Sinica

基　　金：四川省青年基金(04ZQ026-009);四川应用基础项目;四川省国际合作项目;国家科技支撑计划项目(2006BAD01A02)

摘　　要：【目的】研究人工合成小麦中6+8、1.5+10HMW-GS对小麦主要品质性状的影响。【方法】选用一份含6+8和1.5+10HMW-GS的人工合成六倍体小麦Syn-CD780,与栽培品种川育12-1(CY12-1)(亚基组合为1、7+8、2+12)杂交,构建F8重组近交系群体(RIL),分别在3个环境下种植(G05、G06、J06),测定16个品质参数。【结果】(1)2个亲本之间除FY之外,其他品质性状均存在显著差异。籽粒和蛋白质参数(TW、GH、GPC、FY、WGC、SED等)的群体均值在G06、J06环境下介于亲本之间;粉质仪参数表现不一,群体均值或处于亲本之间(FWA、SOF)或亲本范围之外(DST、BRT、QN、NS、LOV等)。(2)所有品质性状在不同亚基组合之间均存在显著差异,尤其是GH、SED和大部分粉质仪参数。同一HMW-GS编码位点上不同等位变异之间的差异,因品质参数而异,并受其它位点的影响。多数品质性状6+8优于7+8,1.5+10与2+12差异不明显。(3)不同亚基组合对小麦品质影响较大,LOV和BS以(1、6+8、1.5+10)最高;NS以(1、7+8、1.5+10)最高。NS与FN呈正相关,与其它品质参数的相关性因试验地点不同而存在较大差异;LOV与SOF呈显著负相关、与多数品质参数呈显著正相关。【结论】来自人工合成六倍体小麦中的HMW-GS6+8优于普通小麦中的7+8,1.5+10则依品质参数和亚基组合方式而异。含HMW-GS6+8和1.5+10的人工合成小麦在普通小麦品质改良上具有一定潜力。[Objective] This study was conducted to determine the effect of 6＋8 and 1.5＋10 HMW-GS on main quality parameters in synthetic hexaploid wheat. [Method] A set of RILs derived from the cross between a synthetic hexaploid wheat with 6＋8 and 1.5＋10 HMW-GS and cultivar Chuanyul2-i （CY12-1） with 1, 7＋8, 2＋12 was planted in three environments in 2005 and 2006. A total of 16 quality parameters were tested. [Result] Significant difference between parents was observed for all quality parameters except flour yield. The mean values of the RILs were intermediate to the parents for grain and protein parameters and some Farinograph parameters （FWA, SOF）, but beyond parents at DST, BRT, QN, NS, LOV. There was a significant difference among combinations of HMW-GS in all quality traits, especially in GH, SED, and most of Farinograph parameters. The difference between alleles at same locus was both influenced by quality parameter and other two loci. For most parameters tested, 6＋8 was better than 7＋8 while there was no difference between 1.5＋10 and 2＋12. End-use quality was greatly influenced by combinations of HMW-GS as the combination of 1, 6＋8, 1.5＋10 had the highest LOV and BS, and the combination of 1, 7＋8 and 1.5＋10 with the highest NS. NS displayed a positive linear relationship with FN and its relationships with other quality parameters were based on experimental environments. LOV had a significantly negative relationship with SOF and positive associations with most of aualitv parameters. [ Conclusion ] HMW-GS 6＋8 in synthetics showed better overall quality characteristics than 7＋8. The effect of 1.5＋ 10 on quality varies with quality parameters and combinations of HMW-GS. Synthetics with subunits 6＋8 and 1.5＋10 have potential application value in end-use quality improvement of common wheat.

关 键 词：人工合成小麦 重组近交系 6+8亚基 1.5+10亚基 HMW-GS 亚基组合 品质性状 

分 类 号：S512.1[农业科学—作物学] TS255.5[轻工技术与工程—农产品加工及贮藏工程]