紫外分光光度法测定鹰嘴豆和豆芽中异黄酮含量的研究  

Determination of Isoflavones in Extracts of Cicer afiefinum L. and Bean Sprout by Ultraviolet Spectrophotometry

作　　者：程珍[1] 阿依古力·阿不列孜 马庆玲[1] 高艳华[1] 阿吉艾克拜尔·艾萨[1] 

机构地区：[1]中国科学院新疆理化技术研究所,新疆乌鲁木齐830011 [2]新疆药品检验所,新疆乌鲁木齐830002

出　　处：《时珍国医国药》2008年第11期2612-2613,共2页Lishizhen Medicine and Materia Medica Research

基　　金：国家科技攻关计划-西部开发项目(No.2005BA901A22)

摘　　要：目的建立一种检测鹰嘴豆异黄酮含量的快速分析方法，并检测鹰嘴豆和豆芽中异黄酮的含量。方法采用紫外分光光度法，检测波长为260．9nm，测定鹰嘴豆及其豆芽中异黄酮的含量测定。结果鹰嘴豆芽素A在2—30μg／ml范围内吸光度与浓度呈良好的线性关系，以黄酮总含量测定结果重复性RSD=1．95％，加样回收率为100．18％。结论为鹰嘴豆提取物及制品提供一种简单、可靠、有效的测定方法。Objective To develop a method for fast determination of isoflavones in Cicer afiefinum L and bean sprout. Methods It was determined by UV spectrophotometry, and the wavelength was 260.9nm. Results The concentration of biochanin A showed a good linear relationship within the range of 30 μg/ml. The recovery rate was 100.18% , RSD = 1.7%. Conclusion The method is simple, reliable and effective.

关 键 词：鹰嘴豆 豆芽 异黄酮 紫外分光光度 

分 类 号：R291.5[医药卫生—民族医学]